Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis.
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis.
复制标题
土拉弗朗西斯菌巨噬细胞生长位点 A (MglA) 蛋白的克隆、表达、纯化、结晶和初步 X 射线衍射分析。
DOI：
10.1107/s1744309110009711
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ji,Xinhua
中科院分区：
文献类型：
--
作者：
Subburaman,Priadarsini;Austin,BrianP;Shaw,GaryX;Waugh,DavidS;Ji,Xinhua
土拉弗朗西斯菌是一种潜在的生物武器，它会在人类和动物中引起一种称为兔热病的罕见传染病。来自土拉弗氏菌的巨噬细胞生长位点 A (MglA) 蛋白与 RNA 聚合酶结合，正向调节其在巨噬细胞内生存和复制所需的多种毒力因子的表达。 MglA 蛋白在大肠杆菌中过量产生、纯化并结晶。这些晶体在阿贡国家实验室先进光子源处衍射至 7.5 Å 分辨率，属于六方空间群 P61 或 P65，晶胞参数 a = b = 125，c = 54 Å。
Francisella tularensis, a potential bioweapon, causes a rare infectious disease called tularemia in humans and animals. The macrophage growth locus A (MglA) protein from F. tularensis associates with RNA polymerase to positively regulate the expression of multiple virulence factors that are required for its survival and replication within macrophages. The MglA protein was overproduced in Escherichia coli, purified and crystallized. The crystals diffracted to 7.5 Å resolution at the Advanced Photon Source, Argonne National Laboratory and belonged to the hexagonal space group P61 or P65, with unit-cell parameters a = b = 125, c = 54 Å.