INFLUENCE OF GASTRIC-INHIBITORY POLYPEPTIDE (GIP) AND GLUCOSE ON THE REGULATION OF GLUCAGON-SECRETION BY PANCREATIC ALPHA-CELLS
INFLUENCE OF GASTRIC-INHIBITORY POLYPEPTIDE (GIP) AND GLUCOSE ON THE REGULATION OF GLUCAGON-SECRETION BY PANCREATIC ALPHA-CELLS
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DOI：
10.1016/0167-0115(91)90035-f
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发表时间：
1991-02-01
影响因子：
--
通讯作者：
GO, VLW
中科院分区：
文献类型：
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作者：
OPARA, EC;GO, VLW
在灌流显微分离的小鼠胰岛中研究了葡萄糖和GIP对胰高血糖素分泌的影响。 使用胰高血糖素特异性抗体30 K，通过放射免疫测定法测定每隔1分钟收集的流出液样品中的胰高血糖素水平。 当葡萄糖浓度分别在5.5 mM和11.1 mM之间交替变化时，20 min内分泌的胰高血糖素总量（7740 +/- 212 pg vs 8630 +/- 36 pg，n = 10）无显著差异。 然而，22.2 mM葡萄糖显著抑制胰高血糖素分泌。 高葡萄糖对胰高血糖素释放的抑制作用被GIP的存在部分但显著地逆转，因为胰高血糖素分泌从单独的22.2mM葡萄糖下不可检测的水平增加到10，175 +/-145pg，n = 10（P < 0.01）。 GIP的促胰高血糖素效应在2.10（-9）-2.10（-7）M范围内呈剂量依赖性，葡萄糖浓度为11.1 mM。 我们的数据显示，GIP能够基本上逆转高葡萄糖负荷对胰高血糖素分泌的抑制作用。
The effects of glucose and GIP on glucagon secretion were studied in perifused microdissected murine pancreatic islets. Glucagon levels were determined in effluent samples collected at 1-min intervals by radioimmunoassay using the glucagon-specific antibody, 30 K. There was no significant difference in the total amount (7740 +/- 212 pg vs 8630 +/- 36 pg, n = 10) of glucagon secreted over a 20 min period when the glucose concentration was alternately shifted between 5.5 mM and 11.1 mM, respectively. However, 22.2 mM glucose profoundly suppressed glucagon secretion. The suppressive effect of high glucose on glucagon release was partially, yet significantly, reversed by the presence of GIP, as glucagon secretion increased from a non-detectable level at 22.2 mM glucose alone to 10,175 +/- 145 pg, n = 10 (P < 0.01). The glucagonotropic effect of GIP was dose-dependent in the range of 2.10(-9)-2.10(-7) M, at 11.1 mM glucose. Our data show that GIP is able to substantially reverse the suppressive effect of a high glucose load on glucagon secretion.