Adult brain retains the potential to generate oligodendroglial progenitors with extensive myelination capacity.
Adult brain retains the potential to generate oligodendroglial progenitors with extensive myelination capacity.
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DOI：
10.1073/pnas.96.7.4089
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发表时间：
1999-03
影响因子：
11.1
通讯作者：
Su Zhang;Bin Ge;Ian D. Duncan
中科院分区：
文献类型：
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作者：
Su Zhang;Bin Ge;Ian D. Duncan
动物中枢神经系统（CNS）病灶区域的再髓鞘形成可以通过神经胶质细胞移植来实现，但这些细胞在人类中用于治疗髓鞘疾病的来源目前仅限于胎儿组织。多能前体细胞存在于成体、胚胎和新生动物的中枢神经系统中，并能分化为谱系受限的祖细胞，如少突胶质祖细胞（OPs）。成人中存在的OPs具有与早期生活中所见的不同的表型，并且它们在中枢神经系统修复中的潜在作用仍然未知。为了深入了解操纵这些前体细胞和/或祖细胞成髓鞘能力的潜力，我们从成年大鼠室管膜下组织分离的神经前体细胞中培养了OPs。表型特征表明，这些OPs类似于新生儿OPs而不是成人OPs，并在移植后产生强大的髓磷脂。因此，从成人大脑中产生这种细胞的能力为探索这些细胞修复成人髓鞘疾病的潜力开辟了一条途径。
Remyelination of focal areas of the central nervous system (CNS) in animals can be achieved by transplantation of glial cells, yet the source of these cells in humans to similarly treat myelin disorders is limited at present to fetal tissue. Multipotent precursor cells are present in the CNS of adult as well as embryonic and neonatal animals and can differentiate into lineage-restricted progenitors such as oligodendroglial progenitors (OPs). The OPs present in adults have a different phenotype from those seen in earlier life, and their potential role in CNS repair remains unknown. To gain insights into the potential to manipulate the myelinating capacity of these precursor and/or progenitor cells, we generated a homogenous culture of OPs from neural precursor cells isolated from adult rat subependymal tissues. Phenotypic characterization indicated that these OPs resembled neonatal rather than adult OPs and produced robust myelin after transplantation. The ability to generate such cells from the adult brain therefore opens an avenue to explore the potential of these cells for repairing myelin disorders in adulthood.