Rapid and sensitive LC-ESI-MS of gangliosides
Rapid and sensitive LC-ESI-MS of gangliosides
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DOI：
10.1016/j.jchromb.2013.11.025
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发表时间：
2014-02-01
影响因子：
3
通讯作者：
Mechref, Yehia
中科院分区：
文献类型：
--
作者：
Garcia, Aldo D.;Chavez, Jorge L.;Mechref, Yehia
神经节苷脂是一类鞘脂，其特征在于神经酰胺脂质链连接到阴离子寡糖部分，其复杂性基于唾液酸化水平而变化。神经节苷脂寡糖链中的杂环化是单糖结构、含量、序列和连接的直接结果。神经节苷脂高度集中在中枢神经系统中，并且是细胞类型特异性的以及发育依赖性的，并且它们的数量和种类在细胞分化期间可以经历剧烈的变化。神经节苷脂的特异性定位还允许与各种生物效应物相互作用，包括糖蛋白、抗体、肽激素和生长因子。目前没有能够鉴定和定量神经节苷脂的快速分析测定。本研究的目的是建立一个可靠的色谱质谱分析的基础上，能够分析复杂的生物样品中的神经节苷脂水平在高灵敏度。我们在这里描述了一种色谱方法，使用氨基柱上的分离是基于亲水性相互作用与糖部分的神经节苷脂。几种神经节苷脂，包括GM 1 -3，GD 1（a，B），GD 2 -3，和GT 1（a，B），在不到10分钟内有效分离，检测限范围为10-50 pg的柱上，浓度动态范围超过4个数量级。所开发的方法允许灵敏的定量神经节苷脂来自不同的食管疾病，包括腺癌，高度异型增生，和巴雷特的患者的血清。(C)2013爱思唯尔有限公司版权所有。
Gangliosides are a class of sphingolipids characterized by a ceramide lipid chain attached to an anionic oligosaccharide moiety that varies in complexity based on the level of sialylation. Heterogeneity in the oligosaccharide chain of gangliosides is a direct result of the monosaccharide structure, content, sequence, and connections. Gangliosides are highly concentrated in the central nervous system, and are cell type-specific as well as development-dependent and their quantities and species can undergo drastic changes during cell differentiation. Specific localization of gangliosides also allows for interaction with a variety of bioeffectors, including glycoproteins, antibodies, peptide hormones, and growth factors. There are currently no rapid analytical assays capable of identifying and quantifying gangliosides. The aim of this study is to establish a reliable chromatographic mass spectrometry based assay capable of profiling ganglioside levels in complex biological samples at high sensitivity. We describe here a chromatographic method using an amino column on which the separation is based on hydrophilic interaction with the sugar moiety of gangliosides. Several gangliosides, including GM1-3, GD1(a,b), GD2-3, and GT1(a,b), were efficiently separated in less than 10 min at a limit of detection ranging between 10-50 pg on column with a concentration dynamic range extending over 4 orders of magnitude. The developed method allowed the sensitive quantitation of gangliosides derived from the blood serum of patients with different esophagus diseases, including, adenocarcinoma, high-grade dysplasia, and Barrett's. (C) 2013 Elsevier B.V. All rights reserved.