Labeling of the neurons of origin of zinc-containing pathways by intraperitoneal injections of sodium selenite.
Labeling of the neurons of origin of zinc-containing pathways by intraperitoneal injections of sodium selenite.
复制标题
通过腹膜内注射亚硒酸钠标记含锌途径起源的神经元。
DOI：
10.1016/0306-4522(90)90076-g
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Frederickson,CJ
中科院分区：
文献类型：
--
作者：
Slomianka,L;Danscher,G;Frederickson,CJ
Intraperitoneal injections of sodium selenite result in the formation of zinc-selenium complexes in zinc-containing axonal boutons (“Timm stainable boutons”), and the zinc-selenium precipitate can be rendered visible in histological sections by silver enhancement. In this work we present evidence, in the rat, that zinc-selenium precipitates formedin vivoafter intraperitoneal injections of sodium selenite are translocated by colchicine-sensitive retrograde transport to neural perikarya when animals are allowed to survive 12–24 h after the selenite administration. Silver enhancement renders the perikaryal precipitates visible and thus demonstrates the perikarya of all zinc-containing neurons in the CNS simultaneously. Large populations of zinc-containing neurons identified by the method are found in layers II, III, and VI of all neocortical areas, in the superficial and deep layers of the prepyriform areas and, with a high degree of regional differentiation, in the retrosplenial, entorhinal, para- and presubicular cortices, the hippocampal formation and the amygdaloid complex. Zinc-containing cells were absent from the caudate-putamen, nucleus accumbens and septal complex. Labeled zinc-containing cells are absent in non-telencephalic parts of the brain. The findings indicate that the zinc-containing circuitry of the brain mainly serves in telencephalic information processing.
