RESPIRATORY BURST OF CIRCULATING POLYMORPHONUCLEAR LEUKOCYTES AND PLASMA ELASTASE LEVELS IN PATIENTS WITH INFLAMMATORY BOWEL-DISEASE IN REMISSION
RESPIRATORY BURST OF CIRCULATING POLYMORPHONUCLEAR LEUKOCYTES AND PLASMA ELASTASE LEVELS IN PATIENTS WITH INFLAMMATORY BOWEL-DISEASE IN REMISSION
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DOI：
10.1007/bf02088341
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发表时间：
1994-03-01
影响因子：
3.1
通讯作者：
BARBARA, L
中科院分区：
文献类型：
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作者：
GIONCHETTI, P;CAMPIERI, M;BARBARA, L
以超氧阴离子自由基生成和粒细胞弹性蛋白酶释放为指标，检测溃疡性结肠炎(10例)、克罗恩病(9例)缓解期患者和对照组(10例)外周血中多形核白细胞的活化情况。用佛波酯刺激细胞后，用分光光度法检测细胞中铁细胞色素的还原，以确定超氧阴离子自由基的产生。采用固相酶免疫测定法测定血浆弹性蛋白酶浓度，作为α1-蛋白酶抑制剂的络合物。溃疡性结肠炎和克罗恩病患者外周血中多形核白细胞产生的超氧化物歧化酶显著低于对照组[中位数(中位数)nmoL/分钟/mg蛋白：克罗恩病7.8(7.1~9.6)；溃疡性结肠炎8.25(7.4~10.3)；对照组14.7(13.6~15.8)](P<；0.001)。相比之下，溃疡性结肠炎和克罗恩病患者的血浆弹性蛋白酶水平与对照组相似。炎症性肠病缓解期患者在无脱颗粒改变的情况下，中性粒细胞呼吸爆发的缺陷可能是这些疾病发病的一个重要因素。
The activation of circulating polymorphonuclear leukocytes was determined in terms of superoxide radical generation and granulocyte elastase release in untreated patients with ulcerative colitis (N = 10) and Crohn's disease (N = 9) in remission and in control subjects (N = 10). Superoxide radical generation was determined by monitoring spectrophotometrically the reduction of ferricytochrome, after stimulation of cells with phorbol myristate acetate. Plasma elastase concentration was measured by a solid-phase enzyme immunoassay technique as the complex with alpha-1-proteinase inhibitor. Superoxide formation by polymorphonuclear leukocytes from patients with ulcerative colitis and Crohn's disease was significantly lower compared with controls [median (range) nmol/min/mg protein: Crohn's disease 7.8 (7.1-9.6); ulcerative colitis 8.25 (7.4-10.3); controls 14.7 (13.6-15.8)] (P < 0.001), while no difference was found between the two groups of patients. In contrast plasma elastase levels in patients with ulcerative colitis and Crohn's disease were similar to that of controls. This defective respiratory burst of polymorphonuclear leukocytes in patients with inflammatory bowel disease in remission, in absence of an altered degranulation, could represent an important factor for the pathogenesis of these diseases.