Key cytomembrane ABC transporters of Saccharomyces cerevisiae fail to improve the tolerance to D-limonene

Key cytomembrane ABC transporters of Saccharomyces cerevisiae fail to improve the tolerance to D-limonene
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酿酒酵母关键细胞膜ABC转运蛋白未能提高对D-柠檬烯的耐受性

DOI:
10.1007/s10529-012-0931-6
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发表时间:
2012-08-01
影响因子:
2.7
通讯作者:
Chen, Jian
Chen, Jian
中科院分区:
工程技术4区
文献类型:
--
作者:
Hu, Feifei;Liu, Jidong;Chen, Jian

文献摘要

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ATP-binding cassette transporters (ABC) are important detoxification proteins and were proposed to play important roles in monoterpene resistance in Saccharomyces cerevisiae. In this work, the transcriptional levels of typical ABC transporters of S. cerevisiae under 85 mg d-limonene/l were evaluated using real-time quantitative PCR. Only the transcriptional level of PDR5, YOR1 and PDR15 were upregulated but overexpression of these genes in S. cerevisiae failed to improve d-limonene tolerance suggesting that other mechanisms are involved in tolerance of yeast to monoterpenes.