Ultrastructure of glomerular basement membrane by quick-freeze and deep-etch methods.
Ultrastructure of glomerular basement membrane by quick-freeze and deep-etch methods.
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速冻和深蚀法研究肾小球基底膜超微结构。
DOI：
10.1038/ki.1991.79
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发表时间：
1991
影响因子：
19.6
通讯作者：
Shinichi Ohno
中科院分区：
文献类型：
--
作者：
H. Takami;A. Naramoto;Hidekazu Shigematsu;Shinichi Ohno
用快速冷冻和深蚀刻复制法对大鼠肾小球基底膜进行了高分辨率的三维观察。中间层(致密层)由6~10 nm的纤维组成，形成网状结构。原纤维之间的间隙呈多边形。纤维间距平均长径为17 nm，短径为13 nm。在外层(rara外板)，纤维与中层的网状结构垂直连接足细胞。在内层(内层)，类似的垂直纤维也将内皮细胞与中间层的网状结构连接在一起。这是首次在原位显示中间层(致密层)的三维网络结构的报告。在外层垂直排列的纤维中，提示足细胞对致密层的锚定作用。快速冷冻-深蚀刻法可用于分析肾小球内的丝状超微结构，并将用于阐明肾脏疾病的病理超微结构。
The glomerular basement membrane of rat kidneys were three-dimensionally observed by quick-freeze and deep-etch replica methods at high resolution. The middle layer (lamina densa) was composed of 6 to 10 nm fibrils which formed a meshwork structure. The space between the fibrils had polygonal shape. The average long dimension of the space between fibrils was 17 nm and the short one was 13 nm. At the outer layer (lamina rara externa), fibrils connected podocytes perpendicularly with the meshwork of the middle layer. At the inner layer (lamina rara interna), similar perpendicular fibrils also connected endothelial cells with the meshwork of the middle layer. This is the first report to visualize the three-dimensional meshwork structure of the middle layer (the lamina densa) in situ. The function of anchoring podocytes to the lamina densa was suggested in the perpendicularly arranged fibrils of the outer layer. The quick-freeze and deep-etch method is useful in analyzing filamentous ultrastructure in glomeruli, and will be applied to clarifying pathological ultrastructure in kidney diseases.
DOI：
10.1002/aja.1001810403
发表时间：
1988
期刊：
The American journal of anatomy
影响因子：
--
作者：
Inoue,S;Leblond,CP
通讯作者：
Leblond,CP
影响因子：
--
作者：
LEBLOND, CP;INOUE, S
通讯作者：
INOUE, S