Impacts of root symbiotic associations on interspecific variation in sugar exudation rates and rhizosphere microbial communities: a comparison among four plant families
Impacts of root symbiotic associations on interspecific variation in sugar exudation rates and rhizosphere microbial communities: a comparison among four plant families
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DOI：
10.1007/s11104-015-2703-2
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发表时间：
2016-02
期刊：
影响因子：
4.9
通讯作者：
A. Okubo;Motomu Matsusaka;S. Sugiyama
中科院分区：
文献类型：
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作者：
A. Okubo;Motomu Matsusaka;S. Sugiyama
根瘤菌和丛枝菌根真菌（AMF）之间的共生关系影响植物根系碳分配的变化，并改变其宿主植物中信号分子的分泌。在这项研究中，我们研究了细菌和真菌共生协会对植物根系糖分泌速率变化和根际微生物群落结构的影响，通过比较四个植物科（例如，Asteraceae，菊科，豆科，禾本科）与不同的细菌和真菌共生associations.Methods和resultsWe确定的23种植物的糖渗出率的回归渗出的糖对根质量通过定期采样的植物生长在盆。根据28种植物根际细菌和真菌群落结构的排序得分，对PCR-DGGE分析的带型进行了评价。植物科是种间糖分泌速率和微生物群落结构变异的最重要来源。与其他植物科相比，紫穗槐科植物缺乏根共生关系，其糖分泌率最低，根际微生物质量最低，真菌群落结构明显。Fabaceae有一个不同的细菌群落结构相对于其他plant family.ConclusionsThese结果表明，根共生协会有很大的影响，糖渗出率和自由生活的微生物群落，栖息在根际。
Background and aimsSymbiotic associations between rhizobia and arbuscular mycorrhyzal fungi (AMF) influence changes in carbon allocation to plant roots and alter the exudation of signal molecules in their host plants. In this study, we examined the impacts of bacterial and fungal symbiotic associations on the variation in sugar exudation rates from plant roots, and in rhizosphere microbial community structure, by comparing four plant families (e.g., Asteraceae, Brassicaceae, Fabaceae, and Poaceae) with different bacterial and fungal symbiotic associations.Methods and resultsWe determined sugar exudation rates of 23 plant species by regressions of exuded sugars against root mass through periodic samplings of plants grown in pots. Banding patterns from PCR-DGGE analyses of 28 plant species were evaluated by ordination scores relative to their rhizosphere bacterial and fungal community structures. Plant family was the most important source of variation in sugar exudation rates and microbial community structure between plant species. Brassicaceae, which lacks a root symbiotic association, had the lowest sugar exudation rate, the lowest rhizosphere microbial mass, and a distinct fungal community structure relative to the other plant families. Fabaceae had a different bacterial community structure relative to the other plant families.ConclusionsThese results demonstrate that root symbiotic associations have great impacts on the rate of sugar exudation and free-living microbial communities that inhabit the rhizosphere.