Multi-component HPLC fingerprinting of radix salviae miltiorrhizae and its LC-MS-MS identification
Multi-component HPLC fingerprinting of radix salviae miltiorrhizae and its LC-MS-MS identification
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DOI：
10.1248/cpb.53.677
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发表时间：
2005-06-01
影响因子：
1.7
通讯作者：
Jiang, ZH
中科院分区：
文献类型：
--
作者：
Hu, P;Liang, QL;Jiang, ZH
采用反相高效液相色谱（HPLC）和液相色谱-质谱联用（LC-MS-MS）技术，对中药中药材丹参的多组分指纹图谱进行了研究。提取物含有水溶性酚类化合物和非极性二萜类化合物，已知是草药的主要生物活性成分，通过两步提取程序制备。采用梯度洗脱模式和280 nm波长的光电二极管阵列检测，建立了能同时分离这两种化合物的高效液相色谱指纹图谱方法。采用LC-MS-MS技术对指纹图谱中的18个峰进行了结构鉴定。大多数丹酚酸的电喷雾质谱（ESI）表现为丹参素和咖啡酸部分的损失，而丹参酮的电喷雾质谱（ESI）表现为水的损失，这与电子电离质谱（ESI - ms）的破碎模式有很大的不同。7批药材的HPLC指纹图谱显示出相似的分离模式，提供了药材中药理活性成分的丰富化学信息，为药材的鉴别和质量评价提供了依据。
The multi-component fingerprinting method of Radix Salviae Miltiorrhizae (Root of Salvia miltiorrhiza B-GE.), an important and popular medicinal herb in traditional Chinese medicine, was studied using reverse-phase HPLC and LC-MS-MS. Extract containing both the water-soluble phenolic compounds and nonpolar diterpenoid compounds known to be the herb's main bioactive components was prepared by a two-step extractive procedure. An HPLC fingerprinting method which can simultaneously separate these two types of compounds was established with gradient elution mode and photodiode array detection at 280 nm. Eighteen peaks in this HPLC fingerprint were structurally identified by employing LC-MS-MS techniques. The electrospray ionization (ESI) MS-MS spectra of most salvianolic acids displayed a characteristic behavior of loss of danshensu and caffeic acid moieties, while those of tanshinones showed a particular behavior of loss of H2O which is quite different from the fragmentation pattern in electron ionization mass spectrometry (EI-MS). The HPLC fingerprints of 7 batches of crude drugs showed similar separation pattern and provided much chemical information of the pharmacologically-active compounds in the crude drugs, which is useful for the authentication and quality evaluation of this medicinal herb.