Resolving confounding enrichment kinetics due to overlapping resonance signals from 13C-enriched long chain fatty acid oxidation and uptake within intact hearts.
Resolving confounding enrichment kinetics due to overlapping resonance signals from 13C-enriched long chain fatty acid oxidation and uptake within intact hearts.
复制标题
解决由于完整心脏内富含 13C 的长链脂肪酸氧化和摄取的共振信号重叠而导致的混杂富集动力学。
DOI：
10.1002/mrm.25446
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发表时间：
2015
影响因子：
3.3
通讯作者：
Lewandowski,EDouglas
中科院分区：
文献类型：
--
作者：
O'Donnell,JMichael;Fasano,MatthewJ;Lewandowski,EDouglas
PurposeLong chain fatty acid (LCFA) oxidation measurements in the intact heart from13C‐NMR rely on detection of13C‐enriched glutamate. However, progressive increases in overlapping resonance signal from LCFA can confound detection of the glutamate 4‐carbon (GLU‐C4) signal. We evaluated alternative13C labeling for exogenous LCFA and developed a simple scheme to distinguish kinetics of LCFA uptake and storage from oxidation.MethodsSequential13C‐NMR spectra were acquired from isolated rat hearts perfused with13C LCFA and glucose. Spectra were evaluated from hearts supplied: U13C LCFA, [2,4,6,8,10,12,14,16‐13C8] palmitate, [2,4,6,8,10,12,14,16,18‐13C9] oleate, [4,6,8,10,12,14,16‐13C7] palmitate, or [4,6,8,10,12,14,16,18‐13C8] oleate.Results13C signal reflected the progressive enrichment at 34.6 ppm from GLU‐C4, confounded by additional signal with distinct kinetics attributed to13C‐enriched LCFA 2‐carbon (34.0 ppm). Excluding13C at the 2‐carbon of both palmitate and oleate eliminated signal overlap and enabled detection of the exponential enrichment of GLU‐C4 for assessing LCFA oxidation.ConclusionEliminating enrichment at the 2‐carbon of13C LCFA resolved confounding kinetics between GLU‐C4 and LCFA 2‐carbon signals. With this enrichment scheme, oxidation of LCFA, the primary fuel for cardiac ATP synthesis, can now be more consistently examined in whole organs with dynamic mode, proton‐decoupled13C‐NMR. Magn Reson Med 74:330–335, 2015. © 2014 Wiley Periodicals, Inc.
