Alternative promotion of the mouse acyl-CoA synthetase 6 (mAcsl6) gene mediates the expression of multiple transcripts with 5′-end heterogeneity:: genetic organization of mAcsl6 variants
Alternative promotion of the mouse acyl-CoA synthetase 6 (mAcsl6) gene mediates the expression of multiple transcripts with 5′-end heterogeneity:: genetic organization of mAcsl6 variants
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DOI：
10.1016/j.bbrc.2004.11.141
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发表时间：
2005-02-04
影响因子：
3.1
通讯作者：
Ryoo, ZY
中科院分区：
文献类型：
--
作者：
Lee, EJ;Kim, HC;Ryoo, ZY
我们报道了小鼠酰基辅酶a合成酶6 （mAcsl6）基因的四个变体和不同的启动子使用。这些变异在小鼠11号染色体上被组织成26个外显子和25个内含子，横跨55kb的DNA，根据它们的5'- utr和外显子13的选择性剪接进行分类。核苷酸序列比对表明，mAcsl6变异1 （mAcsl6_v1）和mAcsl6_v2使用不同的启动子，剪接模式与mAcsl6_v3和mAcsl6_v4不同。启动子分析结果表明mAcsl6启动子1 （mAcsl6_pr1）区域具有负调控功能。为了验证这一结果，我们构建了包含启动子区域mAcsl6_pr1和2以及嵌合转录物的id载体结构。虽然mAcsl6_pr1区域被删除，但mAcsl6-v1和2转录本的检测结果一致。(C) 2004爱思唯尔公司版权所有。
We report four variants and alternative promoter usage for the mouse acyl-CoA synthetase 6 (mAcsl6) gene. The variants, which were organized into 26 exons and 25 introns spanning 55 kb of DNA on mouse chromosome 11, were classified according to their 5'-UTRs and alternative splicing of exon 13. Alignment of the nucleotide sequences showed that the mAcsl6 variant 1 (mAcsl6_v1) and mAcsl6_v2 used a different promoter and had different splicing patterns than mAcsl6_v3 and mAcsl6_v4. The results of the promoter analysis suggest that the mAcsl6 promoter 1 (mAcsl6_pr1) region has a negative regulatory function. To verify this result, we constructed id vector constructs that contained the promoter regions mAcsl6_pr1 and 2, and the chimeric transcript. Although the mAcsl6_pr1 region was deleted, the mAcsl6-v1 and 2 transcripts were detected consistently. (C) 2004 Elsevier Inc. All rights reserved.