Calcyclin is an early vasopressin-induced gene in the renal collecting duct - Role in the long term regulation of ion transport
Calcyclin is an early vasopressin-induced gene in the renal collecting duct - Role in the long term regulation of ion transport
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DOI：
10.1074/jbc.m112435200
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发表时间：
2002-07-12
影响因子：
4.8
通讯作者：
Blot-Chabaud, M
中科院分区：
文献类型：
--
作者：
Courtois-Coutry, N;Le Moellic, C;Blot-Chabaud, M
精氨酸加压素（AVP）在肾脏中的长期作用涉及未知基因的转录。通过对RCCD 1皮质集合管细胞系进行的消减杂交实验，我们确定了钙周期蛋白作为早期AVP诱导的基因（1小时）。钙周期蛋白是一种钙结合蛋白，参与细胞内信号的转导。在肾脏中，钙周期蛋白定位于mRNA水平。在肾小球中，沿着集合管，以及在乳头内衬的上皮中。在RCCD细胞和m-IMCD 3内髓集合管细胞中，钙周期蛋白在细胞质中可见。AVP处理使RCCD 1（4 h时为1.7倍）和m-IMCD 3（7.5 h时为2倍）细胞中Caleyelin mRNA水平逐渐增加。在RCCD 1细胞中，钙周期蛋白蛋白水平增加4小时的AVP处理。在体内，治疗的遗传加压素缺乏的Brattleboro大鼠AVP 4天诱导钙周期蛋白和水通道蛋白-2 mRNA表达的增加。最后，通过透化质膜向RCCD 1细胞中引入抗钙周期蛋白抗体防止了AVP诱导的短路电流的长期（但不是短期）增加。两者合计，这些结果表明，钙周期素是一个早期的加压素诱导的基因，参与在跨上皮离子转运的激素反应的后期阶段。
Long-term effects of arginine vasopressin (AVP) in the kidney involve the transcription of unidentified genes. By subtractive hybridization experiments performed on the RCCD1 cortical collecting duct cell line, we identified calcyclin as an early AVP-induced gene (1 h). Calcyclin is a calcium-binding protein involved in the transduction of intracellular signals. In the kidney, calcyclin was localized at the mRNA level. in the glomerulus, all along the collecting duct, and in the epithelium lining the papilla. In RCCD, cells and in m-IMCD3 inner medullary collecting duct cells, calcyclin was evidence in the cytoplasm. Caleyelin mRNA levels were progressively increased by AVP treatment in RCCD1 (1.7-fold at 4 h) and m-IMCD3 (2-fold at 7.5 h) cells. In RCCD1 cells, calcyclin protein levels were increased by 4 h of AVP treatment. In vivo, treatment of genetically vasopressin-deficient Brattleboro rats with AVP for 4 days induced an increase in both calcyclin and aquaporin-2 mRNA expression. Finally, introduction of anti-calcyclin antibodies into RCCD1 cells by permeabilizing the plasma membrane prevented the long-term (but not short-term) increase in short-circuit current induced by AVP. Taken together, these results suggest that calcyclin is an early vasopressin-induced gene that participates in the late phase of the hormone response in transepithelial ion transport.