Investigation on silent bacterial infections in specimens from pregnant women affected by spontaneous miscarriage
Investigation on silent bacterial infections in specimens from pregnant women affected by spontaneous miscarriage
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DOI：
10.1002/jcp.26952
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发表时间：
2019-01-01
影响因子：
5.6
通讯作者：
Martini, Fernanda
中科院分区：
文献类型：
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作者：
Contini, Carlo;Rotondo, John C.;Martini, Fernanda
流产是妊娠期发生的主要并发症之一。不良妊娠结局与隐性细菌感染之间的关系研究甚少。应用聚合酶链反应（PCR）方法对自然流产女性（SA, n=100）和自愿终止妊娠女性（VI, n=100）的绒毛膜绒毛组织和外周血单个核细胞（PBMCs）进行了细小脲原体和脲原体、生殖支原体和人原体以及沙眼衣原体DNA序列的检测。在SA和VI中分别检测到14%和15%的U. parvum DNA， SA和VI的细菌DNA平均载量分别为1.3 × 10（-1）个拷贝/细胞和2.8 × 10（-3）个拷贝/细胞；在SA和VI样品中分别检测到3%和2%的urealiticum DNA， SA样品的平均DNA载量为3.3 × 10（-3）拷贝/细胞，VI样品的平均DNA载量为1.6 × 10（-3）拷贝/细胞；5% DNA载量为1.3 × 10（-4）拷贝/细胞的SA标本和6% DNA载量为1.4 × 10（-4）拷贝/细胞的VI标本中检测到古人类支原体DNA；在DNA载量为1.5 × 10（-4）拷贝/细胞的SA标本中检测到沙眼衣原体DNA的比例为3%，在平均DNA载量为1.4 × 10（-4）拷贝/细胞的VI标本中检测到沙眼衣原体DNA的比例为4%。SA组和VI组外周血检出脲原体、支原体和沙眼衣原体dna，检出率为1% ~ 3%。首次采用实时荧光定量PCR （qPCR）技术对SA和VI患者的绒毛膜绒毛组织和PBMCs中的细菌进行了研究。这些数据可能有助于了解SA沉默感染的作用和我们的知识。
Miscarriage is one of the main complications occurring in pregnancy. The association between adverse pregnancy outcomes and silent bacterial infections has been poorly investigated. Ureaplasma parvum and urealiticum, Mycoplasma genitalium and hominis and Chlamydia trachomatis DNA sequences have been investigated by polymerase chain reaction (PCR) methods in chorionic villi tissues and peripheral blood mononuclear cells (PBMCs) from females with spontaneous abortion (SA, n=100) and females who underwent voluntary interruption of pregnancy (VI, n=100). U. parvum DNA was detected in 14% and 15% of SA and VI, respectively, with a mean of bacterial DNA load of 1.3 x 10(-1) copy/cell in SA and 2.8 x 10(-3) copy/cell in VI; U. urealiticum DNA was detected in 3% and 2% of SA and VI specimens, respectively, with a mean DNA load of 3.3 x 10(-3) copy/cell in SA and 1.6 x 10(-3) copy/cell in VI; M. hominis DNA was detected in 5% of SA specimens with a DNA load of 1.3 x 10(-4) copy/cell and in 6% of VI specimens with a DNA load of 1.4 x 10(-4) copy/cell; C. trachomatis DNA was detected in 3% of SA specimens with a DNA load of 1.5 x 10(-4) copy/cell and in 4% of VI specimens with a mean DNA load of 1.4 x 10(-4) copy/cell. In PBMCs from the SA and VI groups, Ureaplasma spp, Mycoplasma spp and C. trachomatis DNAs were detected with a prevalence of 1%-3%. Bacteria were investigated, for the first time, by quantitative real-time PCR (qPCR) in chorionic villi tissues and PBMCs from women affected by SA and VI. These data may help to understand the role and our knowledge of the silent infections in SA.