LATS2-Ajuba complex regulates γ-tubulin recruitment to centrosomes and spindle organization during mitosis
LATS2-Ajuba complex regulates γ-tubulin recruitment to centrosomes and spindle organization during mitosis
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DOI：
10.1016/j.febslet.2005.12.096
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发表时间：
2006-02-06
期刊：
影响因子：
3.5
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Abe, Y;Ohsugi, M;Yamamoto, T
LATS 2是果蝇属肿瘤抑制因子lats/warts的人类同源物，并编码有丝分裂激酶，其生理作用仍有待阐明。我们进行了酵母双杂交筛选，并确定了一个LIM蛋白Ajuba，作为LATS 2的结合伴侣。LATS 2定位于整个细胞周期的中心体，并在有丝分裂期间与筋骨草相关，有助于后者的有丝分裂磷酸化。LATS 2或Ajuba的耗尽损害了有丝分裂开始时γ-微管蛋白的中心体积累和纺锤体形成，表明LATS 2-Ajuba复合物通过将γ-微管蛋白募集到中心体来调节纺锤体装置的组织。(c)2006年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
LATS2 is a human homolog of Drosaphila tumor suppressor lats/warts, and encodes a mitotic kinase whose physiological roles remain to be elucidated. We performed yeast two-hybrid screening and identified a LIM protein Ajuba, as a binding partner of LATS2. LATS2 was localized to the centrosomes throughout the cell cycle and was associated with Ajuba during mitosis, contributing to latter's mitotic phosphorylation. Depletion of LATS2 or Ajuba impaired centrosomal accumulation of gamma-tubulin and spindle formation at the onset of mitosis, suggesting that the LATS2-Ajuba complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.