Comparison of Escherichia coli Strains as Agents for Bacterial Interference
Comparison of Escherichia coli Strains as Agents for Bacterial Interference
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DOI：
10.1086/653070
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发表时间：
2010-06-01
影响因子：
4.5
通讯作者：
Hull, Richard A.
中科院分区：
文献类型：
--
作者：
Darouiche, Rabih O.;Riosa, Sarah;Hull, Richard A.
Because antimicrobial-based measures have limited efficacy in preventing symptomatic catheter-associated urinary tract infection (UTI) and could be associated with adverse effects, the role of bacterial interference is being increasingly examined. 1 Several clinical studies reported that deliberate bladder inoculation with Escherichia coli 83972, a strain associated with asymptomatic bacteriuria, may reduce the frequency of symptomatic UTI in certain patient groups. 2'3 In vitro studies showed that E. coli 83972 can colonize urinary catheters and can interfere with subsequent catheter colonization by various uropathogens. 4Although it is considered to be a strain of low virulence and, to our knowledge, it has not been reported to cause symptomatic UTI, E. coli 83972 contains an intact pap gene cluster. 5 Therefore, it retains the potential for expressing a P tissue adhesion that is a fimbrial hemagglutinin associated with uropathogenic E. coli. 6 Although the P adherence phenotype is not expressed by E. coli 83972 under normal growth conditions in vitro or while growing in the urine of colonized subjects, the pap-83972 gene cluster is fully functional and directs expression of a hemagglutinin with pap-2 (class III) adherence phenotype when it is expressed from a recombinant plasmid. 7 E. coli HU2117 is a papGA derivative of E. coli83972 that has lost the genetic potential for expressing P-specific adherence. 8 Although the efficacy and safety of E. coli 83972 have been assessed in many clinical trials, 2'3, 8 only one pilot trial reported that coating of bladder catheters with E. coli HU2117 can establish asymptomatic bacteriuria without causing symptomatic UTI. 9 The purpose of this study was to evaluate the utility of E. coli HU2117 as a potentially safer alternative than E. coli 83972 for future human studies of catheter colonization.
