Isolation and characterisation of an aryl-β-D-glucoside uptake and utilisation system (abg) from the gram-positive ruminal Clostridium species C-longisporum
Isolation and characterisation of an aryl-β-D-glucoside uptake and utilisation system (abg) from the gram-positive ruminal Clostridium species C-longisporum
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DOI：
10.1007/s004380050641
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Thomson, JA
中科院分区：
文献类型：
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作者：
Brown, GD;Thomson, JA
从瘤胃梭菌（“C. longisporum”）。该系统由三个基因abgG、abgF和abgA以及许多调控区组成，包括abgG和abgF基因之前的终止子/抗终止子类型茎环结构。这些基因编码的蛋白质的相似性分析表明，它们负责通过抗终止（AbgG）、芳基-β-葡萄糖苷（AbgF）的摄取和磷酸化以及胞内磷酸化糖苷（AbgA）的水解来调节abg系统。获得了AbgF和AbgA功能的实验证据。虽然不可能证明AbgG的任何功能，但鉴定了abgG基因5'端的启动子，其负责下游基因的表达。abg系统在编码和非编码调控区与革兰氏阴性肠杆菌科的操纵子非常相似。
A phosphotransferase-dependent aryl-beta-glucoside uptake and utilisation system (abg) was isolated from the ruminal Clostridium ("C. longisporum"). The system is composed of three genes, abgG, abgF and abgA, and a number of regulatory regions, including terminator/antiterminator type stem-loop structures preceding the abgG and abgF genes. Similarity analysis of the proteins encoded by these genes indicated that they were responsible for the regulation of the abg system through antitermination (AbgG), the uptake and phosphorylation of aryl-beta-glucosides (AbgF) and the hydrolysis of the intracellular phosphorylated glycosides (AbgA). Experimental evidence for the functions of AbgF and AbgA was obtained. Although it was not possible to demonstrate any function for AbgG, a promoter 5' to the abgG gene was identified which was responsible for expression of the downstream genes. The abg system is remarkably similar to operons from the gram negative Enterobacteriaceae, both in the coding and non-coding regulatory regions.