Analysis of 5S rDNA Organization and Variation in Polyploid Hybrids from Crosses of Different Fish Subfamilies
Analysis of 5S rDNA Organization and Variation in Polyploid Hybrids from Crosses of Different Fish Subfamilies
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不同鱼类亚科杂交多倍体杂种的5S rDNA组织及变异分析
DOI：
10.1002/jez.b.21346
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发表时间：
2010-07-15
影响因子：
2.2
通讯作者：
Liu, Yun
中科院分区：
文献类型：
--
作者：
Qin, Qinbo;He, Weiguo;Liu, Yun
本文对红鲫、团头鲂及其多倍体后代的5S和NTS基因进行了序列分析。RCC的三个单体5S rDNA类别（指定为I类：203 bp; II类：340 bp;和III类：477 bp）的特征在于不同的NTS类型（分别指定为83、220和357 bp单体的NTS-I、II和III）。在BSB中，仅观察到一个单体5S rDNA（指定为IV类：188 bp），其特征在于一个NTS类型（指定为NTS-IV：68 bp）。在多倍体后代中，四倍体（4 nRB）杂种部分地继承了其母本（RCC）的5S rDNA类别;然而，它们也具有独特的5S rDNA序列（命名为I-L类：203 bp）和新的NTS序列（命名为NTS-I-L：83 bp）。没有观察到特征性的父系5S rDNA序列（IV类）。三倍体（3 nRB）杂种的5SrDNA完全遗传自亲本物种，并且基本上保留了亲本的5SrDNA结构组织。这些结果首次揭示了多倍性对鱼类5S rDNA多基因家族的组织和进化的影响，也有助于阐明脊椎动物基因组进化的各个方面。J. Exp. Zool.（Mol. Dev. Evol.）314 B：403-411，2010. (C)2010 Wiley-Liss，Inc.
In this article, sequence analysis of the coding region (5S) and adjacent nontranscribed spacer (NTS) were conducted in red crucian carp (RCC), blunt snout bream (BSB), and their polyploid offspring. Three monomeric 5S rDNA classes (designated class I: 203 bp; class II: 340 bp; and class III: 477 bp) of RCC were characterized by distinct NTS types (designated NTS-I, II, and III for the 83, 220, and 357 bp monomers, respectively). In BSB, only one monomeric 5S rDNA was observed (designated class IV: 188 bp), which was characterized by one NTS type (designated NTS-IV: 68 bp). In the polyploid offspring, the tetraploid (4nRB) hybrids partially inherited 5S rDNA classes from their female parent (RCC); however, they also possessed a unique 5S rDNA sequence (designated class I-L: 203 bp) with a novel NTS sequence (designated NTS-I-L: 83 bp). The characteristic paternal 5S rDNA sequences (class IV) were not observed. The 5S rDNA of triploid (3nRB) hybrids was completely inherited from the parental species, and generally preserved the parental 5S rDNA structural organization. These results first revealed the influence of polyploidy on the organization and evolution of the multigene family of 5S rDNA of fish, and are also useful in clarifying aspects of vertebrate genome evolution. J. Exp. Zool. (Mol. Dev. Evol.) 314B:403-411, 2010. (C) 2010 Wiley-Liss, Inc.