Transcriptome profile of murine gammaherpesvirus-68 lytic infection
Transcriptome profile of murine gammaherpesvirus-68 lytic infection
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DOI：
10.1099/vir.0.18639-0
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Nash, AA
中科院分区：
文献类型：
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作者：
Ebrahimi, B;Dutia, BM;Nash, AA
鼠γ疱疹病毒-68基因组编码73个蛋白编码开放阅读框，与人γ（2）疱疹病毒以及独特的基因和细胞同源物具有广泛的相似性。我们进行了转录组分析的阶段特异性病毒RNA在允许感染使用的阿托伐他汀为基础的微阵列。使用这种方法，M4，K3，ORF 38，ORF 50，ORF 57和ORF 73被指定为基于放线菌酮处理的立即早期基因。微阵列分析还鉴定了10个具有早期表达动力学的转录物，32个具有早期-晚期表达动力学的转录物和29个具有晚期表达动力学的转录物。后一组主要由结构蛋白组成，并且相对于其他病毒转录物显示出高表达水平。此外，我们检测到所有八个tRNA样转录在放线菌酮和膦酰基乙酸的存在下。用MHV-68裂解感染也导致DNA芯片中包含的细胞转录物的表达显著降低。这种病毒转录本分析的全球方法提供了一个强大的系统，用于检查与疾病发病机制相关的裂解和潜伏病毒感染之间的分子转换。
The murine gammaherpesvirus-68 genome encodes 73 protein-coding open reading frames with extensive similarities to human gamma(2) herpesviruses, as well as unique genes and cellular homologues. We performed transcriptome analysis of stage-specific viral RNA during permissive infection using an oligonucleotide-based microarray. Using this approach, M4, K3, ORF38, ORF50, ORF57 and ORF73 were designated as immediate-early genes based on cycloheximide treatment. The microarray analysis also identified 10 transcripts with early expression kinetics, 32 transcripts with early-late expression kinetics and 29 transcripts with late expression kinetics. The latter group consisted mainly of structural proteins, and showed high expression levels relative to other viral transcripts. Moreover, we detected all eight tRNA-like transcripts in the presence of cycloheximide and phosphonoacetic acid. Lytic infection with MHV-68 also resulted in a significant reduction in the expression of cellular transcripts included in the DNA chip. This global approach to viral transcript analysis offers a powerful system for examining molecular transitions between lytic and latent virus infections associated with disease pathogenesis.