Nonlinear optical microscopy for drug delivery monitoring and cancer tissue imaging
Nonlinear optical microscopy for drug delivery monitoring and cancer tissue imaging
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DOI：
10.1002/jrs.2622
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发表时间：
2010-08
影响因子：
2.5
通讯作者：
R. Mouras;Grigore Rischitor;A. Downes;Donald Salter;A. Elfick
中科院分区：
文献类型：
--
作者：
R. Mouras;Grigore Rischitor;A. Downes;Donald Salter;A. Elfick
开发了一种结合相干反斯托克斯拉曼散射（汽车）、双光子激发荧光（TPEF）、二次谐波产生（SHG）和和频率产生（SFG）的多模态非线性光学显微镜，并将其应用于乳腺癌组织和MCF-7细胞的成像以及活细胞中抗癌药物的监测。TPEF成像显示药物优先定位于耐药细胞的细胞质和核膜。此外，通过弹性蛋白的自发荧光产生的TPEF信号和来自乳腺组织切片中胶原纤维的SHG信号观察细胞外基质。此外，来自蛋白质和（PO 2）−的汽车信号允许识别肿瘤。无标记成像与癌细胞和组织的重要组成部分的化学对比表明，多模态非线性光学显微镜的早期检测和诊断癌症的潜力。版权所有© 2010约翰威利父子有限公司.
A multimodal nonlinear optical microscope that combines coherent anti-Stokes Raman scattering (CARS), two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and sum-frequency generation (SFG) was developed and applied to image breast cancer tissue and MCF-7 cells as well as monitoring anticancer drug delivery in live cells. TPEF imaging showed that drugs are preferentially localized in the cytoplasm and the nuclear envelope in resistant cells. Moreover, the extracellular matrix was observed by TPEF signals arising from elastin's autofluorescence and SHG signals from collagen fibrils in breast tissue sections. Additionally, CARS signals arising from proteins and (PO2)− allowed identification of tumors. Label-free imaging with chemical contrast of significant components of cancer cells and tissue suggests the potential of multimodal nonlinear optical microscopy for early detection and diagnosis of cancer. Copyright © 2010 John Wiley & Sons, Ltd.