Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.
Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.
复制标题
使用代谢物分析快速筛选重组毕赤酵母菌株中的细胞应激反应。
DOI：
10.1007/s10295-017-1904-5
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发表时间：
2017-03
影响因子：
3.4
通讯作者：
Bundy JG
中科院分区：
文献类型：
--
作者：
Tredwell GD;Aw R;Edwards-Jones B;Leak DJ;Bundy JG
酵母毕赤酵母中的杂合蛋白质生产可受到对高表达水平的生物学响应的限制;未折叠蛋白质响应（UPR）是该生物体中蛋白质生产成功的关键决定因素。在这里，我们使用了一些不同的重组菌株的非靶向NMR代谢谱（代谢组学），以适合筛选级实验的小型化形式进行。我们鉴定了许多代谢产物（来自细胞提取物和上清液），其与UPR相关基因转录物良好相关，因此可能是未来大量巴斯德毕赤酵母克隆的高通量筛选的潜在生物标志物。本文的在线版本（doi：10.1007/s10295-017-1904-5）包含补充材料，可供授权用户使用。
Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels; the unfolded protein response (UPR) is a key determinant of the success of protein production in this organism. Here, we used untargeted NMR metabolic profiling (metabolomics) of a number of different recombinant strains, carried out in a miniaturized format suitable for screening-level experiments. We identified a number of metabolites (from both cell extracts and supernatants) which correlated well with UPR-relevant gene transcripts, and so could be potential biomarkers for future high-throughput screening of large numbers of P. pastoris clones. The online version of this article (doi:10.1007/s10295-017-1904-5) contains supplementary material, which is available to authorized users.