Silver Nanoparticles Decrease the Viability of Cryptosporidium parvum Oocysts
Silver Nanoparticles Decrease the Viability of Cryptosporidium parvum Oocysts
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DOI：
10.1128/aem.02806-15
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发表时间：
2016-01-01
影响因子：
4.4
通讯作者：
Bridle, Helen
中科院分区：
文献类型：
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作者：
Cameron, Pamela;Gaiser, Birgit K.;Bridle, Helen
水生原生动物寄生虫微小隐孢子虫的卵囊对氯消毒具有高度抵抗力。我们在这里表明，银纳米粒子（AgNPs）和银离子显着降低卵囊活力，在剂量依赖性的方式，浓度为0.005和500 μ g/ml之间，通过脱囊试验和壳/子孢子比进行评估。对于脱囊百分比，对于500 μ g/ml的AgNP，结果是统计学显著的，从对照的83%降低到AgNP的33%。对于Ag离子，结果在500和5，000 μ g/ml时具有统计学显著性，但脱囊百分比值仅分别从对照的86%降低至66%和62%。子孢子/壳比在AgNP暴露后受到更大程度的影响，可能是因为子孢子通过与NP的相互作用而被破坏。我们还通过高光谱成像证明了存在双重相互作用模式，Ag离子进入卵囊并破坏子孢子，而AgNP与细胞壁相互作用，并且在高浓度下能够完全打破卵囊壁。
Oocysts of the waterborne protozoan parasite Cryptosporidium parvum are highly resistant to chlorine disinfection. We show here that both silver nanoparticles (AgNPs) and silver ions significantly decrease oocyst viability, in a dose-dependent manner, between concentrations of 0.005 and 500 mu g/ml, as assessed by an excystation assay and the shell/sporozoite ratio. For percent excystation, the results are statistically significant for 500 mu g/ml of AgNPs, with reductions from 83% for the control to 33% with AgNPs. For Ag ions, the results were statistically significant at 500 and 5,000 mu g/ml, but the percent excystation values were reduced only to 66 and 62%, respectively, from 86% for the control. The sporozoite/shell ratio was affected to a greater extent following AgNP exposure, presumably because sporozoites are destroyed by interaction with NPs. We also demonstrated via hyperspectral imaging that there is a dual mode of interaction, with Ag ions entering the oocyst and destroying the sporozoites while AgNPs interact with the cell wall and, at high concentrations, are able to fully break the oocyst wall.