Comparative Genomic Analysis of Malaria Mosquito Vector-Associated Novel Pathogen Elizabethkingia anophelis
Comparative Genomic Analysis of Malaria Mosquito Vector-Associated Novel Pathogen Elizabethkingia anophelis
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DOI：
10.1093/gbe/evu094
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发表时间：
2014-01-01
影响因子：
3.3
通讯作者：
Yang, Liang
中科院分区：
文献类型：
--
作者：
Teo, Jeanette;Tan, Sean Yang-Yi;Yang, Liang
在过去的十年中，重症监护病房（ICU）中的嗜酸粒细胞增多。由于缺乏有效的治疗方案，嗜酸粒细胞增多症感染的治疗具有挑战性，导致高死亡率。长期以来，嗜沙金杆菌感染被归因于脑膜炎败血症嗜沙金杆菌。最近，我们利用全基因组测序技术揭示了E。按蚊是两个重症监护病房发生的嗜斯金菌爆发的病原体。我们对7株医院分离的大肠杆菌进行了比较基因组分析。按蚊菌株与5种可用的嗜斯金原虫属（Escherichkingia spp.）保存在国家生物技术信息数据库中心的基因组。应用泛基因组方法鉴定了黄菊属的核心基因组和泛基因组。我们发现，与医院分离的病原体E。脑膜炎败血症ATCC 12535菌株是医院分离的E.按蚊菌株具有与E.按蚊Ag1和R26菌株分离自疟疾蚊子载体冈比亚按蚊的中肠微生物群。本文报道了黄喉盘菌的核心基因组和辅助基因组。具有赋予抗生素抗性和毒性的基因。我们的研究强调E.按蚊是医院环境中出现的细菌病原体。
Acquisition of Elizabethkingia infections in intensive care units (ICUs) has risen in the past decade. Treatment of Elizabethkingia infections is challenging due to the lack of effective therapeutic regimens, leading to a high mortality rate. Elizabethkingia infections have long been attributed to Elizabethkingia meningoseptica. Recently, we used whole-genome sequencing to reveal that E. anophelis is the pathogenic agent for an Elizabethkingia outbreak at two ICUs. We performed comparative genomic analysis of seven hospital-isolated E. anophelis strains with five available Elizabethkingia spp. genomes deposited in the National Center for Biotechnology Information Database. A pan-genomic approach was applied to identify the core- and pan-genome for the Elizabethkingia genus. We showed that unlike the hospital-isolated pathogen E. meningoseptica ATCC 12535 strain, the hospital-isolated E. anophelis strains have genome content and organization similar to the E. anophelis Ag1 and R26 strains isolated from the midgut microbiota of the malaria mosquito vector Anopheles gambiae. Both the core- and accessory genomes of Elizabethkingia spp. possess genes conferring antibiotic resistance and virulence. Our study highlights that E. anophelis is an emerging bacterial pathogen for hospital environments.