ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ENTEROCOCCI - RESULTS OF A SURVEY CONDUCTED BY THE UNITED-KINGDOM-NATIONAL-EXTERNAL-QUALITY-ASSESSMENT-SCHEME-FOR-MICROBIOLOGY
ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ENTEROCOCCI - RESULTS OF A SURVEY CONDUCTED BY THE UNITED-KINGDOM-NATIONAL-EXTERNAL-QUALITY-ASSESSMENT-SCHEME-FOR-MICROBIOLOGY
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DOI：
10.1093/jac/32.3.401
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发表时间：
1993-09-01
影响因子：
5.2
通讯作者：
GEORGE, R
中科院分区：
文献类型：
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作者：
SNELL, JJS;BROWN, DFJ;GEORGE, R
英国国家微生物外部质量评估计划向参与者分发了六种肠球菌，要求测试它们对氨苄西林/青霉素和万古霉素的敏感性以及对庆大霉素的高水平耐药性。超过98%的实验室正确地报告了耐药菌株对青霉素和氨苄西林的耐药性。然而，敏感菌株的相关性较差，只有1-6%和69-83%的实验室报告这些菌株对青霉素和氨苄西林敏感。那些使用5-25μg氨苄西林纸片或断点方法的实验室报告的敏感菌株的结果比使用2μg纸片的实验室报告的结果更正确。与使用金黄色葡萄球菌NCTC 6571(牛津菌株)的参与者相比，使用颜色和肠球菌作为对照的参与者和根本不使用对照的参与者更有可能正确报告氨苄西林敏感菌株。对于万古霉素敏感株和高水平万古霉素耐药株，报告的正确率分别为93-96%和96-99%。对于低水平的万古霉素耐药株，只有50%-54%的报告是正确的。与使用10、20、25、30或100μg纸片的参与者相比，使用5μg万古霉素纸片的参与者正确报告了更多的低水平耐药菌株。与使用肠球菌ORS的参与者相比，没有使用对照的参与者报告低水平耐药菌株的比例更高。AreusNCFC6571作为对照。对于高水平的庆大霉素耐药(HLGR)菌株，96-98%的报告是正确的。根据菌株的不同，12%到41%的参与者也报告了对非HLGR菌株的高水平耐药性。那些使用低含量纸片(≤30μg)和断点或最低抑菌浓度方法的人比使用高含量纸片的人更有可能报告非HLGR的菌株为HLGR。那些使用肠球菌作为对照的人比使用任何一种方法的人更不可能报告不是HLGR的菌株为HLGR。Areus NCTC 6571矿石。Colias是一个对照，而那些不使用对照。
Six strains of enterococci were distributed to participants in the United Kingdom National External Quality Assessment Scheme for Microbiology with a request that they be tested for susceptibility to ampicillin/penicillin and vancomycin and for high-level resistance to gentamicin. More than 98% of laboratories correctly reported the resistant strains as resistant to penicillin and ampicillin. However, there was a poorer correlation for the susceptible strains, with only 1–6% and 69–83% of laboratories reporting the strains as susceptible to penicillin and ampicillin respectively. Those laboratories which used 5–25 μg ampicillin discs or breakpoint methods reported proportionally more results correctly for the susceptible strains than those using 2 μg discs. Participants usingEscherichia colior an enterococcus as a control and those not using a control at all were more likely to report ampicillin-susceptible strains correctly than those usingStaphylococcus aureusNCTC 6571 (Oxford strain). For vancomycin-susceptible and high-level vancomycin-resistant strains, 93–96% and 96–99% of reports respectively were correct. For the low-level vancomycin-resistant strains, only 50–54% of reports were correct. Participants using a 5 μg vancomycin disc reported proportionally more of the low-level resistant strains correctly than those using 10, 20, 25, 30 or 100 μg discs. Participants not using controls were proportionally more likely to report the low-level resistant strains as susceptible than those using an enterococcus orS. aureusNCFC 6571 as a control. For the high-level gentamicin-resistant (HLGR) strains, 96–98% of reports were correct. Depending on the strain, between 12% and 41% of participants also reported high-level resistance for the strains which were not HLGR. Those using low-content discs (≤30 μg) and breakpoint or MIC methods were more likely to report strains which were not HLGR as HLGR than those using high-content discs. Those using an enterococcus as a control were less likely to report strains which were not HLGR as HLGR than those using eitherS. aureusNCTC 6571 orE. colias a control and those not using a control.