Differential expression of TGF beta 1, beta 2 and beta 3 genes during mouse embryogenesis.
Differential expression of TGF beta 1, beta 2 and beta 3 genes during mouse embryogenesis.
复制标题
DOI：
--
复制
发表时间：
1991
期刊：
影响因子：
4.6
通讯作者：
P. Schmid;D. Cox;G. Bilbe;R. Maier;Mcmaster Gary Kent
中科院分区：
文献类型：
--
作者：
P. Schmid;D. Cox;G. Bilbe;R. Maier;Mcmaster Gary Kent
我们通过 Northern 分析和原位杂交检查了小鼠胚胎发生过程中 TGF β 1、β 2 和 β 3 的表达。 TGF beta 1 主要在胚胎的中胚层成分中表达，例如胚胎。胎儿肝脏的造血细胞和卵黄囊的造血岛、几个内脏器官的间质组织和骨化骨组织。在早期面部间充质以及一些内胚层和外胚层上皮细胞层（例如肺和耳蜗上皮细胞）中发现了最强的 TGF beta 2 信号。 TGFβ3 在椎前组织、一些间皮细胞和肺上皮细胞中最强。所有三种亚型均在骨组织中表达，但在空间和时间上表现出不同的表达模式。在须毛囊的根鞘中，TGFβ1、β2和β3同时表达。我们讨论了这些结果对 TGF β 基因及其受体的已知调控元件的影响。
We have examined by Northern analysis and in situ hybridisation the expression of TGF beta 1, beta 2 and beta 3 during mouse embryogenesis. TGF beta 1 is expressed predominantly in the mesodermal components of the embryo e.g. the hematopoietic cells of both fetal liver and the hemopoietic islands of the yolk sac, the mesenchymal tissues of several internal organs and in ossifying bone tissues. The strongest TGF beta 2 signals were found in early facial mesenchyme and in some endodermal and ectodermal epithelial cell layers e.g., lung and cochlea epithelia. TGF beta 3 was strongest in prevertebral tissue, in some mesothelia and in lung epithelia. All three isoforms were expressed in bone tissues but showed distinct patterns of expression both spatially and temporally. In the root sheath of the whisker follicle, TGF beta 1, beta 2 and beta 3 were expressed simultaneously. We discuss the implication of these results in regard to known regulatory elements of the TGF beta genes and their receptors.