Epithelial cell differentiation pathways in the human prostate: Identification of intermediate phenotypes by keratin expression
Epithelial cell differentiation pathways in the human prostate: Identification of intermediate phenotypes by keratin expression
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DOI：
10.1177/002215540104900214
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发表时间：
2001-02-01
影响因子：
3.2
通讯作者：
Masters, JRW
中科院分区：
文献类型：
--
作者：
Hudson, DL;Guy, AT;Masters, JRW
成年后前列腺生长缓慢，导致良性前列腺增生（BPH），常导致晚年尿道梗阻。前列腺增生的症状是65岁以上男性手术的第二大常见原因。本研究的目的是确定前列腺增生组织中细胞增殖和细胞分化之间的关系。使用多种抗体，同时检测不同的荧光基团共轭二抗，检测上皮细胞的几个亚群。除K14外，基底细胞还以不同的组合表达角蛋白15、17、19，部分管腔细胞也同时表达K19和K8、K18。细胞角蛋白和Ki-67共染色显示，44%的增殖细胞表达K14, 36%表达K19，但差异无统计学意义。该报告详细描述了角蛋白表达与前列腺细胞增殖之间的关系，并指出k19阳性细胞形成了上皮基底层和管腔层之间的联系。
The prostate grows slowly throughout adult life, leading to benign prostatic hyperplasia (BPH), which often results in urethral obstruction in later years. The symptoms of BPH are the second most common reason for surgery in men over 65. The aim of this study was to determine the relationship between cell proliferation and cell differentiation in BPH tissue. Using multiple antibodies, simultaneously detected with different fluorophore-conjugated secondary antibodies, several subpopulations of epithelial cells were detected. In addition to K14, basal cells also expressed keratins 15, 17, and 19 in Various combinations, and some of the luminal cells also expressed K19 together with K8 and K18. Co-staining for cytokeratins and Ki-67 indicated that 44% of proliferative cells expressed K14 and 36% K19, although the difference was not statistically significant. This report provides a detailed description of the relationship between keratin expression and cell proliferation in the prostate and indicates that K19-positive cells form the link between the basal and luminal layers of the epithelium.