SON is a spliceosome-associated factor required for mitotic progression
SON is a spliceosome-associated factor required for mitotic progression
复制标题
DOI：
10.4161/cc.9.13.12151
复制
发表时间：
2010-07-01
期刊：
影响因子：
4.3
通讯作者：
Chen, Junjie
中科院分区：
文献类型：
--
作者：
Huen, Michael S. Y.;Sy, Shirley M. H.;Chen, Junjie
真核RNA剪接机制致力于切除细胞中许多新生RNA转录物上的内含子序列的艰巨任务，并且这样做有助于其转录组的正确翻译。值得注意的是，新出现的证据表明，RNA剪接也可能在维持基因组稳定性方面发挥直接作用。在这里，我们报告的RNA/DNA结合蛋白SON作为剪接体的一个组成部分，在有丝分裂过程中发挥多效性作用的鉴定。我们发现SON对于细胞增殖是必不可少的，并且其失活触发了MAD 2依赖的有丝分裂延迟。此外，SON缺陷伴随有缺陷的染色体聚集、受损的染色体分离和胞质分裂，这反过来又导致细胞非整倍性和细胞死亡。总之，我们的研究揭示了SON和有丝分裂之间的特定联系，并强调了RNA加工作为控制细胞增殖和分裂的额外调节机制的潜力。
The eukaryotic RNA splicing machinery is dedicated to the daunting task of excising intronic sequences on the many nascent RNA transcripts in a cell, and in doing so facilitates proper translation of its transcriptome. Notably, emerging evidence suggests that RNA splicing may also play direct roles in maintaining genome stability. Here we report the identification of the RNA/DNA-binding protein SON as a component of spliceosome that plays pleiotropic roles during mitotic progression. We found that SON is essential for cell proliferation, and that its inactivation triggers a MAD2-dependent mitotic delay. Moreover, SON deficiency is accompanied by defective chromosome congression, compromised chromosome segregation and cytokinesis, which in turn contributes to cellular aneuploidy and cell death. In summary, our study uncovers a specific link between SON and mitosis, and highlights the potential of RNA processing as additional regulatory mechanisms that govern cell proliferation and division.