Proteomic analysis of 3-MCPD and 3-MCPD dipalmitate toxicity in rat testis.
Proteomic analysis of 3-MCPD and 3-MCPD dipalmitate toxicity in rat testis.
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3-MCPD 和 3-MCPD 二棕榈酸酯对大鼠睾丸毒性的蛋白质组学分析。
DOI：
10.1016/j.fct.2015.06.002
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A. Lampen
中科院分区：
文献类型：
--
作者：
S. Sawada;A. Oberemm;T. Buhrke;C. Meckert;C. Rozycki;A. Braeuning;A. Lampen
含脂肪和盐的食品的热处理促进3-氯丙烷-1，2-二醇（3-MCPD）及其脂肪酸酯的形成。暴露于3-MCPD的大鼠出现睾丸病变和Leydig细胞瘤。3-MCPD和3-MCPD酯毒性被认为是由3-MCPD及其代谢产物引起的，因为3-MCPD酯在肠道中水解。糖酵解抑制是3-MCPD毒性的少数已知分子机制之一。为了更深入地了解这一过程，选择了比较蛋白质组学方法，基于对雄性Wistar大鼠的28天重复给药喂养研究。动物接受等摩尔剂量的3-MCPD或3-MCPD二棕榈酸酯。还给予了较低剂量的3-MCPD二棕榈酸酯。无组织病理学变化支持早期细胞紊乱的分析。睾丸进行了分析，然后通过质谱蛋白质鉴定的二维凝胶电泳。数据提供了一个全面的概述3-MCPD和3-MCPD二棕榈酸酯诱导的蛋白质组变化在大鼠睾丸器官损伤的早期阶段。结果与已知的3-MCPD对生殖功能的影响一致，大大扩展了我们对3-MCPD细胞反应的了解，并支持3-MCPD和3-MCPD酯的毒性通过共同效应物介导的假设。DJ-1被确定为3-MCPD暴露的候选标志物。
Thermal treatment of foodstuff containing fats and salt promotes the formation of 3-chloropropane-1,2-diol (3-MCPD) and its fatty acid esters. 3-MCPD-exposed rats develop testicular lesions and Leydig cell tumors. 3-MCPD and 3-MCPD ester toxicity is thought to be caused by 3-MCPD and its metabolites, since 3-MCPD esters are hydrolyzed in the gut. Inhibition of glycolysis is one of the few known molecular mechanisms of 3-MCPD toxicity. To obtain deeper insight into this process, a comparative proteomic approach was chosen, based on a 28-days repeated-dose feeding study with male Wistar rats. Animals received equimolar doses of 3-MCPD or 3-MCPD dipalmitate. A lower dose of 3-MCPD dipalmitate was also administered. Absence of histopathological changes supported an analysis of early cellular disturbance. Testes were analyzed by two-dimensional gel electrophoresis followed by mass-spectrometric protein identification. Data provide a comprehensive overview of proteomic changes induced by 3-MCPD and 3-MCPD dipalmitate in rat testis in an early phase of organ impairment. Results are compatible with known 3-MCPD effects on reproductive function, substantially extend our knowledge about cellular responses to 3-MCPD and support the hypothesis that toxicity of 3-MCPD and 3-MCPD esters is mediated via common effectors. DJ-1 was identified as a candidate marker for 3-MCPD exposure.
影响因子：
3.4
作者：
Steiner SR;Milton E;Philbert MA
通讯作者：
Philbert MA
DOI：
10.1073/pnas.0407708101
发表时间：
2004-11-23
影响因子：
11.1
作者：
Miki, K;Qu, WD;O'Brien, DA
通讯作者：
O'Brien, DA