The effects of jiawei xiaoyao powder on the levels of dopamine and serotonin in hepatocellular carcinoma rats.
The effects of jiawei xiaoyao powder on the levels of dopamine and serotonin in hepatocellular carcinoma rats.
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DOI：
10.16557/j.cnki.1000-7547.2020.02.005
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Xia, M.
中科院分区：
文献类型：
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作者：
Cheng, Jin-lai;Wen, Xiao-yu;Xia, M.
目的：探讨加味逍遥散对肝郁脾虚型肝癌大鼠脑内多巴胺（DA）、5-羟色胺（5-HT）含量的影响。方法：36只SD大鼠随机分为对照组、肝癌模型组和加味采用二乙基亚硝胺（DEN）结合慢性不可预见性温和刺激（CITS）建立肝郁脾虚型肝癌大鼠模型。观察各组大鼠的症状和体征，HE染色观察大鼠肝脏病理变化，高效液相色谱法检测额叶皮质DA和5-HT水平。结果：与对照组比较，HCC组病理表现为肝小叶结构破坏、增生细胞异型增生表现、双核细胞病理核分裂征等，症状积分符合肝郁脾虚证，颅内DA、5-HT明显降低。经加味消瘤丸治疗后，大鼠肝组织病理学病变较轻，症状明显改善，脑DA、5-HT明显升高。结论：加味消痞丸能改善肝郁脾虚大鼠的症状，其机制可能与增加脑内单胺类神经递质有关。
Objective: To investigate the effects of Chinese medicine prescription Jiawei Xiaoyao Powder (JWXYP) on the levels of dopamine (DA) and serotonin (5-HT) in the brain of hepatocellular carcinoma (HCC) rats with liver depression and spleen deficiency. Methods: Thirty-six SD rats were randomly divided into the control group, the HCC model group (HCC) and the flavored JWXYP group (JWXYP). A rat model of HCC with liver depression and spleen deficiency was established by combining diethylnitrosamine (DEN) with chronic unpredictable mild stimulation (CUMS). The symptoms and signs of the rats in each group were observed, the pathological changes of the rats' liver were observed by HE staining, and the DA and 5-HT levels in the frontal cortex were detected by HPLC. Results:Compared with the control group, the pathological manifestations of the HCC group were hepatic lobular structure destruction, dysplastic manifestations of proliferative cells, pathological mitotic signs of binuclear cells,etc, and symptom scores were consistent with liver depression and spleen deficiency syndrome, with a significant decrease in intracranial DA and 5-HT. After JWXYP treatment, the hepatic histopathological malignancies of the rats were mild, the symptoms were significantly improved, and the cerebral DA and 5-HT were significantly increased. Conclusion: JWXYP can improve the symptoms of liver stagnation and spleen deficiency in rats, and the mechanism may be related to the increase of monoamine neurotransmitters in the brain.