Emergence of carbapenem-resistant Pseudomonas alcaligenes and Pseudomonas paralcaligenes clinical isolates with plasmids harbouring blaIMP-1 in Japan
Emergence of carbapenem-resistant Pseudomonas alcaligenes and Pseudomonas paralcaligenes clinical isolates with plasmids harbouring blaIMP-1 in Japan
复制标题
日本出现带有 blaIMP-1 质粒的耐碳青霉烯类产碱假单胞菌和副产碱假单胞菌临床分离株
DOI：
10.1099/jmm.0.001684
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发表时间：
2023
影响因子：
3
通讯作者：
Kirikae Teruo
中科院分区：
文献类型：
--
作者：
Ono Emi;Tohya Mari;Tada Tatsuya;Hishinuma Tomomi;Watanabe Shin;Kuwahara-Arai Kyoko;Kirikae Teruo
产生金属β-内酰胺酶（MBL）的碳青霉烯类耐药假单胞菌的出现已成为世界范围内严重的医学问题。IMP型MBL于1991年在日本首次发现。从那时起，它已成为最常见的MBL类型之一。假设/差距声明。无毒的假单胞菌菌种，如产碱假单胞菌，在临床环境中作为编码碳青霉烯酶的耐药相关基因的储存库。方法。2019年在日本东京的一家医院对碳青霉烯类耐药革兰氏阴性病原体进行了主动监测。在筛选的543份碳青霉烯类耐药菌株中，有2种为假单胞菌。结果：全基因组测序结果表明，前一株分离株为P.产碱假单胞菌（Pseudomonasalcaligenes），与产碱假单胞菌（P.产碱菌两株菌均对所有碳青霉烯类抗生素耐药，并携带编码IMP-1 MBL的blaIMP-1基因，从而对碳青霉烯类抗生素产生耐药。将P.产碱菌和P.质粒pMRCP 2和pMRCP 1333分别含有323125 bp和16592 bp的DNA片段。pMRCP 2的82%序列与P. pMRCP 1333的79%的序列与木糖氧化无色杆菌FDAARGOS 162的质粒序列相似性> 95%。 pMRCP 2和pMRCP 1333的blaIMP-1基因组环境完全不同。产碱菌和P.在医院中，碳青霉烯类耐药基因作为载体和储存库发挥作用。
Introduction.The emergence of carbapenem-resistantPseudomonasspecies producing metallo-β-lactamase (MBL) has become a serious medical problem worldwide. IMP-type MBL was firstly detected in 1991 in Japan. Since then, it has become one of the most prevalent types of MBLs.Hypothesis/Gap statement.Avirulent species ofPseudomonas, such asPseudomonas alcaligenes, function as reservoirs of drug resistance-associated genes encoding carbapenemases in clinical settings.Methodology.Active surveillance for carbapenem-resistant Gram-negative pathogens was conducted in 2019 at a hospital in Tokyo, Japan. Of the 543 samples screened for carbapenem-resistant isolates, 2 were species ofPseudomonas. One was from a stool sample from a medical staff member, and the other was from a stool sample from a hospitalized patient.Results.Whole-genome sequencing showed that the former isolate was a strain ofP. alcaligenes, and the latter was a strain ofPseudomonas paralcaligenes, a species close toP. alcaligenes. Both isolates were resistant to all carbapenems and harbouredblaIMP-1genes encoding IMP-1 MBL, which conferred resistance to carbapenems. TheblaIMP-1genes ofP. alcaligenesandP. paralcaligeneswere located on the plasmids, pMRCP2, 323125 bp in size, and pMRCP1333, 16592 bp in size, respectively. The sequence of 82 % of pMRCP2 was 92 % similar to the sequence of a plasmid ofP. aeruginosaPA83, whereas the sequence of 79 % of pMRCP1333 was >95 % similar to the sequence of a plasmid ofAchromobacter xylosoxidansFDAARGOS 162. The genomic environments surrounding theblaIMP-1of pMRCP2 and pMRCP1333 differed completely from each other.Conclusions.These results indicate that the two isolates acquiredblaIMP-1from different sources and thatP. alcaligenesandP. paralcaligenesfunction as vectors and reservoirs of carbapenem-resistant genes in hospitals.