Functional evaluation of artificial skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique.
Functional evaluation of artificial skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique.
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DOI：
10.1089/ten.tea.2010.0312
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发表时间：
2011
影响因子：
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通讯作者：
Yasunori Yamamoto;A. Ito;H. Fujita;E. Nagamori;Y. Kawabe;M. Kamihira
中科院分区：
文献类型：
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作者：
Yasunori Yamamoto;A. Ito;H. Fujita;E. Nagamori;Y. Kawabe;M. Kamihira
骨骼肌组织工程目前应用于各种研究领域，包括再生医学，药物筛选和生物致动器开发，所有这些都需要仿生和功能性骨骼肌组织的制造。本研究采用磁性阳离子脂质体磁性标记C2 C12成肌细胞，通过外加磁场构建三维人工骨骼肌组织。评估人造组织构建物的骨骼肌功能，例如生化和收缩特性。组织学研究显示，在组织内观察到细长的多核肌管。肌肉特异性标记物，如肌生成素，肌球蛋白重链和原肌球蛋白的表达，通过蛋白质印迹分析检测组织结构。此外，肌酸激酶活性在分化过程中增加。在电脉冲的作用下，人工组织结构收缩以产生物理力（最大抽搐力，33.2 μN [1.06 mN/mm 2]）。测得该组织的基强度和时值分别为4.45 V和0.72 ms。这些结果表明，在本研究中制造的人工骨骼肌组织构建体的生理功能和所获得的数据，其功能特性的评价可能为未来的骨骼肌组织工程研究提供有用的信息。
Skeletal muscle tissue engineering is currently applied in a variety of research fields, including regenerative medicine, drug screening, and bioactuator development, all of which require the fabrication of biomimic and functional skeletal muscle tissues. In the present study, magnetite cationic liposomes were used to magnetically label C2C12 myoblast cells for the construction of three-dimensional artificial skeletal muscle tissues by an applied magnetic force. Skeletal muscle functions, such as biochemical and contractile properties, were evaluated for the artificial tissue constructs. Histological studies revealed that elongated and multinucleated myotubes were observed within the tissue. Expression of muscle-specific markers, such as myogenin, myosin heavy chain and tropomyosin, were detected in the tissue constructs by western blot analysis. Further, creatine kinase activity increased during differentiation. In response to electric pulses, the artificial tissue constructs contracted to generate a physical force (the maximum twitch force, 33.2 μN [1.06 mN/mm2]). Rheobase and chronaxie of the tissue were determined as 4.45 V and 0.72 ms, respectively. These results indicate that the artificial skeletal muscle tissue constructs fabricated in this study were physiologically functional and the data obtained for the evaluation of their functional properties may provide useful information for future skeletal muscle tissue engineering studies.