Sulfonation of Lactobacillus plantarum WLPL04 exopolysaccharide amplifies its antioxidant activities in vitro and in a Caco-2 cell model
Sulfonation of Lactobacillus plantarum WLPL04 exopolysaccharide amplifies its antioxidant activities in vitro and in a Caco-2 cell model
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植物乳杆菌 WLPL04 胞外多糖的磺化可增强其体外和 Caco-2 细胞模型中的抗氧化活性
DOI：
10.3168/jds.2018-15831
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发表时间：
2019-07-01
影响因子：
3.5
通讯作者：
Wei, Hua
中科院分区：
文献类型：
--
作者：
Liu, Zhengqi;Dong, Lingyi;Wei, Hua
系统研究了植物乳杆菌WLPL04胞外多糖(EPS)及其硫酸化EPS的抗氧化活性和对过氧化氢对Caco-2细胞氧化损伤的保护作用。经傅立叶变换红外光谱分析证实，从纯化的EPS中成功地对胞外多糖进行了磺化，磺化度为0.30。通过1，1-二苯基-12-苦基肼、超氧阴离子自由基和羟基自由基清除实验表明，EPS和硫酸化EPS在体外均表现出抗氧化活性，且在1,000µg/mL时其抗氧化活性显著增强。Caco-2细胞存活率在1~100µg/RNL的EPS和硫酸化EPS之间无显著差异。在H_2O_2损伤的Caco-2细胞模型中，EPS和硫酸化EPS均能显著抑制细胞内氧自由基和丙二醛水平的升高，且与相同浓度的纯化EPS(100 mU/L)相比，EPS的抑制作用分别提高了40.86%和61.11%。在抗氧化相关酶(超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶)活性和基因表达(SODS、GPX2、MT1M)方面，EPS和硫酸化EPS均表现出较强的抗氧化能力，且硫酸EPS的抗氧化能力明显高于EPS或对照组。综上所述，磺化是提高植物乳杆菌WLPL04粗多糖体外和Caco-2细胞抗氧化活性的有效策略。
Exopolysaccharide (EPS) of Lactobacillus plantarum WLPL04 and its sulfated EPS were systematically investigated for their antioxidant activities and effects on protecting the oxidative damage of Caco-2 cells from H2O2. Exopolysaccharide was successfully sulfonated from purified EPS as confirmed by Fourier-transform infrared spectroscopy, and the degree of sulfonation was 0.30. Both EPS and sulfated EPS showed antioxidant activities in vitro determined by 1,1-dipheny12-picrylhydrazyl, superoxide, and hydroxyl radical scavenging tests, and those activities of sulfated EPS were significantly enhanced at 1,000 mu g/mL. Cell viabilities of Caco-2 in the range of 1 to 100 mu g/rnL of EPS and sulfated EPS showed no significant difference. In H2O2 -damaged Caco-2 cells models, EPS and sulfated EPS significantly inhibited the enhancement of reactive oxygen species and malondialdehyde levels, and sulfated EPS enhanced the effects by 40.86% and 61.11% when compared with the purified EPS at the same concentration of 100 mu g/mL, respectively. For the activities of antioxidant-related enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and expression of genes (SODS, GPX2, MT1M) on Caco-2 cells, strong protection abilities against the oxidative stress were displayed from both EPS and sulfated EPS, and sulfated EPS exhibited significant enhancement as compared with either EPS or control groups. In summary, sulfonation is an effective strategy for improving the antioxidant activities of EPS from L. plantarum WLPL04 in vitro and on Caco-2 cells.