Homoeologue expression insights into the basis of growth heterosis at the intersection of ploidy and hybridity in Cyprinidae.
Homoeologue expression insights into the basis of growth heterosis at the intersection of ploidy and hybridity in Cyprinidae.
复制标题
同源表达对鲤科倍性和杂交交叉点生长杂种优势基础的见解
DOI：
10.1038/srep27040
复制
发表时间：
2016-06-06
影响因子：
4.6
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Ren L;Li W;Tao M;Qin Q;Luo J;Chai J;Tang C;Xiao J;Tang X;Lin G;Zhang C;Duan W;Hong Y;Liu S
杂交和多倍化被认为是形成新的表观遗传调控的重要驱动力。为了研究杂交和多倍体化过程中基因表达的变化规律，我们采用RNA-seq和qRT-PCR技术研究了鲫（Carassius auratusred var.（R）和鲤鱼（Cyprinus carpio）（C）。通过比较杂交后代与亲本的相对表达水平，定义了肝组织中的表达水平优势（ELD）和同源表达偏倚（HEB）。结果表明，多倍体化有助于同源ELD的转化。杂交对HEB基因表达的影响大于多倍体化，而多倍体化对基因表达的影响大于杂交。同时，在生长相关基因中发现了类似的表达模式。结果表明，杂交和多倍化导致了三种组织（肝脏、肌肉和卵巢）中母体HEB的差异程度。这项研究的结果将增加我们对二倍体杂种和异源四倍体快速生长的潜在调节机制的了解。整体表达和同源表达的差异程度有助于新形成的杂种的生长杂种优势，确保异源四倍体物种形成的持续成功。
Hybridization and polyploidization are considered important driving forces that form new epigenetic regulations. To study the changing patterns of expression accompanying hybridization and polyploidization, we used RNA-seq and qRT-PCR to investigate global expression and homoeologue expression in diploid and tetraploid hybrids ofCarassius auratusred var. (♀) (R) andCyprinus carpio(♂) (C). By comparing the relative expression levels between the hybrids and their parents, we defined the expression level dominance (ELD) and homoeologue expression bias (HEB) in liver tissue. The results showed that polyploidization contributed to the conversion of homoeologue ELD. In addition, hybridization had more effect on the change in HEB than polyploidization, while polyploidization had more effect on the change of global gene expression than hybridization. Meanwhile, similar expression patterns were found in growth-related genes. The results suggested that hybridization and polyploidization result in differential degrees of maternal HEB in three tissues (liver, muscle and ovary) tested. The results of this study will increase our understanding of the underlying regulation mechanism of rapid growth in diploid hybrids and allotetraploids. The differential degrees of global expression and homoeologue expression contribute to growth heterosis in newly formed hybrids, ensuring the on-going success of allotetraploid speciation.