Green tea polyphenols improve isoflurane-induced cognitive impairment via modulating oxidative stress
Green tea polyphenols improve isoflurane-induced cognitive impairment via modulating oxidative stress
复制标题
绿茶多酚通过调节氧化应激改善异氟烷引起的认知障碍
DOI：
10.1016/j.jnutbio.2019.07.004
复制
发表时间：
2019
影响因子：
5.6
通讯作者：
Gu Xiaoping
中科院分区：
文献类型：
--
作者：
Song Yuting;Li Xiuxiu;Gong Xiangdan;Zhao Xin;Ma Zhengliang;Xia Tianjiao;Gu Xiaoping
麻醉暴露可导致学习和记忆障碍，其机制尚不清楚。据报道，绿茶多酚(GTP)具有神经保护作用。本研究旨在探讨GTP对异氟醚诱导的认知功能障碍的治疗作用。取6周龄雄性C57BL/6J小鼠，用1.6%异氟醚处理6h。模型小鼠麻醉前分别给予GTP 25 mg/kg连续7d多次给药和7d单次给药75 mg/kg。采用恐惧条件反射实验和新目标识别实验评估小鼠的认知能力。用试剂盒检测超氧化物歧化酶(SOD)活性。Western印迹法检测右侧海马区p-CaMKII、p-CREB和BDNF的蛋白表达水平。结果表明，异氟醚麻醉6h后3d内出现认知功能障碍。同时，大鼠海马区的超氧化物歧化酶也同步下降。P-CaMKII、p-CREB和BDNF的表达水平也下调。GTP每天25 mg/kg可显著减轻异氟醚麻醉后第3天的认知功能障碍。此外，GTP 25 mg/kg/d可有效缓解异氟醚引起的超氧化物歧化酶、p-CaMKII、p-CREB和BDNF水平的下降。但单次注射75 mg/kg的GTP对小鼠无明显影响。本研究表明，GTP可减轻异氟醚所致的认知功能障碍，其积极作用可能与其抗氧化作用有关。
Anesthetic exposure induces learning and memory impairment and the mechanisms remain unknown. Green tea polyphenols(GTP) have been reported to be neuroprotective. The present study was performed to examine the therapeutic potential of GTP on isoflurane-induced cognitive deficits. Six-week-old male C57BL/6J mice were treated with 1.6% isoflurane for 6 hours. Multiple-dose of GTP at 25 mg/kg for 7 consecutive days and single-dose at 75 mg/kg on the 7th day were respectively administered intraperitoneally to model mice before anesthesia. Fear conditioning test and novel objection recognition were conducted to assess cognition of mice. Superoxide dismutase (SOD) was evaluated using assay kits. Protein expression levels of right hippocampus p-CaMKII, p-CREB and BDNF were examined by Western blot. Our results indicated that 6 h isoflurane anesthesia induced cognitive impairment in early 3 days. Meanwhile, the hippocampus SOD declined in step. The expression levels of p-CaMKII, p-CREB and BDNF were also downregulated. GTP 25mg/kg per day significantly attenuated cognitive dysfunction on Day 3 following isoflurane anesthesia. Moreover, GTP 25mg/kg per day effectively mitigated isodlurane-induced declines of SOD, as well as the p-CaMKII, p-CREB and BDNF levels. However, single-dose at 75 mg/kg of GTP had no significant effects. This study indicated that GTP attenuate isoflurane-induced cognition impairment and this positive effects may be related to its antioxidant properties.