Structure-function relationships in the adipose cell. II. Pinocytosis and factors influencing its activity in the isolated adipose cell.
Structure-function relationships in the adipose cell. II. Pinocytosis and factors influencing its activity in the isolated adipose cell.
复制标题
脂肪细胞中的结构 - 功能关系。 ii。皮细胞增多症和影响其活性在分离的脂肪细胞中的因素。
DOI：
10.1083/jcb.46.2.342
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发表时间：
1970-08
影响因子：
7.8
通讯作者：
Cushman, S W
中科院分区：
文献类型：
--
作者：
Cushman, S W
通过测量胶体金的摄取来检测脂肪细胞中的胞核活性。在所有实验条件下，在分离的脂肪细胞中都有胞饮活性；细胞的部分囊泡成分可以通过电子显微镜鉴定为胞饮起源。分离的脂肪细胞似乎通过吞噬作用摄取血清白蛋白。肾上腺素可增强分离脂肪细胞的吞噬细胞活性，但胰岛素不能。通过同时测量摄取放射性胶体金、U-葡萄糖~(14)C~(14)C~(14)C掺入二氧化碳、总脂、甘油三酯和甘油三酯脂肪酸，以及在无激素和有胰岛素或肾上腺素存在的情况下非酯化脂肪酸的释放，研究了胞吞作用与脂肪细胞代谢活性的关系。激素引起的脂质代谢变化和胞饮活动之间的相关性表明，细胞内非酯化脂肪酸水平是调节细胞胞饮活动及其代谢的一个因素，脂肪细胞的胞饮作用在非酯化脂肪酸的胞外-胞内运输中发挥作用。
Pinocytic activity in the adipose cell has been examined by measuring the uptake of colloidal gold. Pinocytic activity occurs in the isolated adipose cell under all experimental conditions; a portion of the vesicular elements of the cell can be identified by electron microscopy as pinocytic in origin. The isolated adipose cell appears to take up serum albumin by pinocytosis. Pinocytic activity in the isolated adipose cell is enhanced by epinephrine, but not by insulin. The relationship between pinocytosis and the metabolic activity of the adipose cell has been studied by measuring simultaneously the uptake of radioactive colloidal gold, the incorporation of 14C-counts from U-glucose-14C into CO2, total lipid, triglyceride glycerol and triglyceride fatty acids, and the release of nonesterified fatty acids in the absence of hormones and in the presence of insulin or epinephrine. Correlations between hormone-produced alterations in lipid metabolism and in pinocytic activity suggest that intracellular nonesterified fatty acid levels are a factor in the regulation of both the cell's pinocytic activity and its metabolism and that pinocytosis in the adipose cell functions in the extracellular-intracellular transport of nonesterified fatty acids.