Mutagenic activity of surface soil and quantification of 1,3-, 1,6-, and 1,8-dinitropyrene isomers in soil in Japan.
Mutagenic activity of surface soil and quantification of 1,3-, 1,6-, and 1,8-dinitropyrene isomers in soil in Japan.
复制标题
日本表层土壤的诱变活性以及土壤中 1,3-、1,6- 和 1,8-二硝基芘异构体的定量。
DOI：
--
复制
发表时间：
2000
影响因子：
4.1
通讯作者：
K. Wakabayashi
中科院分区：
文献类型：
--
作者：
T. Watanabe;S. Goto;Y. Matsumoto;M. Asanoma;T. Hirayama;N. Sera;Y. Takahashi;O. Endo;S. Sakai;K. Wakabayashi
为了阐明日本非农业表层土壤的致突变潜力，在1996年11月至1997年3月期间从5个不同地理区域收集了110个土壤样品，并通过艾姆斯/沙门氏菌试验对土壤样品的有机提取物进行了检查。在存在和/或不存在哺乳动物代谢活化系统（S9混合物）的情况下，大多数土壤浸提液对菌株TA 98和TA 100均显示出致突变性，表明表层土壤在很大程度上受到环境诱变剂的污染。在海基南、科比和大坂采集的土壤样品对这两种菌株均具有高度致突变性，并且它们对不含S9混合物的TA 98的效力极高，每克土壤诱导超过12 000个回复突变体。另一方面，室兰的土壤样品对含S9混合物的TA 100显示出较强的致突变性。此外，1，3-二硝基芘（DNP），1，6-DNP，1，8-DNP在三个大都市地区的10个采样点采集的土壤样品中进行了定量的荧光检测相应的二氨基芘异构体使用高效液相色谱法（HPLC）。在所有土壤样品中均检测到3种DNP异构体，1，3-、1，6-和1，8-DNP异构体的含量分别为12-3270、14-5587和1,3 -6809 pg/g。海基南表层土壤中DNP三种异构体的总量均大于10 ng/g土壤。在大坂采集的样品中，DNP异构体对土壤提取物致突变性的贡献率最高，三种DNP异构体的贡献率之和约为50%。这些结果表明，表层土壤主要是污染的诱变化合物和DNP异构体是一类主要的诱变和致癌化合物污染表层土壤。
To clarify the mutagenic potential of nonagricultural surface soil in Japan, 110 soil samples were collected from five geographically different areas between November 1996 and March 1997, and organic extracts of the soil samples were examined by the Ames/Salmonella assay. Most of the soil extracts showed mutagenicity toward both strains TA98 and TA100 in the presence and/or absence of a mammalian metabolic activation system (S9 mix), suggesting that surface soil is largely contaminated with environmental mutagens. Soil samples collected at Hekinan, Kobe, and Osaka were highly mutagenic toward both strains, and their potencies toward TA98 without S9 mix were extremely high, inducing more than 12 000 revertants per gram of soil. On the other hand, soil samples from Muroran showed strong mutagenicity toward TA100 with S9 mix. Furthermore, 1, 3-dinitropyrene (DNP), 1,6-DNP, and 1,8-DNP in soil samples collected at 10 sampling sites in three metropolitan areas were quantified by fluorometric detection of the corresponding diaminopyrene isomers using high-performance liquid chromatography (HPLC). Three DNP isomers were detected in all soil samples, and the amounts of 1,3-, 1,6-, and 1,8-DNP isomers in the soil samples were 12-3270, 14-5587, and 13-6809 pg/g, respectively. The gross amount of three DNP isomers in surface soil collected at Hekinan was more than 10 ng per gram of soil. The highest contribution ratios of DNP isomers to the mutagenicity of soil extracts were observed for the samples collected at Osaka, and the total of the contribution ratios of three DNP isomers was about 50%. These results suggest that surface soil is largely contaminated with mutagenic compounds and that DNP isomers are one class of major mutagenic and carcinogenic compounds contaminating surface soil.