POLYPEPTIDE COMPONENTS OF VIRIONS, TOP COMPONENT AND CORES OF REOVIRUS TYPE-3
POLYPEPTIDE COMPONENTS OF VIRIONS, TOP COMPONENT AND CORES OF REOVIRUS TYPE-3
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DOI：
10.1016/0042-6822(69)90017-8
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发表时间：
1969-01-01
期刊：
影响因子：
3.7
通讯作者：
JOKLIK, WK
中科院分区：
文献类型：
--
作者：
SMITH, RE;ZWEERINK, HJ;JOKLIK, WK
呼肠孤病毒3型Dearing株病毒粒子的衣壳蛋白部分由聚丙烯酰胺凝胶电泳确定的7种多肽组成。这些多肽的分子量已估计通过比较其在SDS-含有凝胶的迁移率与已知分子量的标记蛋白。还确定了病毒体中每种多肽的分子数。多肽λ1分别有113和80个分子（M.W. 155，000）和λ2（M.W. 140，000）; 23和550个多肽分子μ1（M.W. 80，000）和μ2（M.W. 72，000）;和多肽σ1（M.W. 42，000），σ2（M.W. 38，000）和σ3（M.W. 34 000人）。此外，病毒体还含有约500个分子的非常小的多肽，其分子量为5000- 10，000。呼肠孤病毒顶部组分（缺乏RNA的颗粒）中的多肽与病毒体中的多肽相同。核心含有衣壳多肽λ1、λ2和σ 2。核心可以类似地从顶部组分制备。核心的最高组分含有与病毒粒子核心相同的多肽，但转化所需的酶处理切割了多肽λ 1。三种大小的衣壳多肽的分子量是这样的，它们可能是由呼肠孤病毒基因组RNA的L、M和S片段转录的单顺反子信使RNA分子编码的。因此，衣壳多肽使用3个L区段中的2个、3个M区段中的2个（或可能仅1个）和4个S区段中的3个中的信息。
The capsid protein moiety of virions of the Dearing strain of reovirus type 3 consists of seven species of polypeptides as determined by polyacrylamide gel electrophoresis. The molecular weights of these polypeptides have been estimated by comparing their rate of migration in SDS-containing gels with that of marker proteins of known molecular weights. The number of molecules of each polypeptide in the virion has also been determined. There are 113 and 80 molecules, respectively, of polypeptidesλ1(M.W. 155,000) andλ2(M.W. 140,000); 23 and 550 molecules, respectively, of polypeptidesμ1(M.W. 80,000) andμ2(M.W. 72,000); and 31, 202, and 890 molecules, respectively, of polypeptidesσ1(M.W. 42,000),σ2(M.W. 38,000), andσ3(M.W. 34,000). In addition virions also contain about 500 molecules of a very small polypeptide, the molecular weight of which is 5000–10,000.The polypeptides in reovirus top component (particles lacking RNA) are identical with those of virions.Treatment with chymotrypsin or trypsin converts virions to cores which lack the outer shell of capsomeres. Cores contain capsid polypeptidesλ1,λ2, andσ2.Cores can similarly be prepared from top component. Top component cores contain the same polypeptides as virion cores, but the enzyme treatment necessary for the conversion cleaves polypeptideλ1.The molecular weights of the three size classes of capsid polypeptides are such that they are probably coded by monocistronic messenger RNA molecules transcribed from the L, M, and S segments of reovirus genome RNA. The capsid polypeptides thus use the information in 2 of the 3 L segments, 2 (or possibly only 1) of the 3 M segments, and 3 of the 4 S segments.