Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates
Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates
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DOI：
10.1016/j.vetmic.2017.04.015
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发表时间：
2017-05-01
影响因子：
3.3
通讯作者：
Cid, Dolores
中科院分区：
文献类型：
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作者：
Garcia-Alvarez, Andres;Isabel Vela, Ana;Cid, Dolores
多杀性巴氏杆菌是一种病原体，可在包括羊和猪在内的广泛宿主中引起疾病。通过MLST（多宿主和RIRDC数据库）和毒力相关基因WAG）分型对来自绵羊肺炎的分离株进行表征，并与猪分离株进行比较。绵羊和猪分离株没有共享任何ST，这两种方案确定，并表现出不同的VAG概况。利用多宿主数据库，在43个绵羊分离株中鉴定出16个ST，其中2个ST（ST 50和ST 19）占分离株的53.5%，在48个猪分离株中鉴定出7种MLST基因型（ST 3、ST 11和ST 62）占分离株的75%。绵羊VAG基因型以tbpA +/toxA +为主（69.8%），猪VAG基因型以pfhA +和hgbB +为主（分别占62.5%和33.3%）。使用RIRDC方案进一步分型通过多宿主方案鉴定的ST的代表性绵羊和猪分离株。在绵羊分离株中鉴定出7株ST（ST 95（RIRDC）、ST 131（RIRDC）、ST 203（RIRDC）、ST 320（RIRDC）、ST 324（RIRDC）、ST 321（RIRDC）和ST 321（RIRDC）），后四种序列类型是本研究中鉴定的新ST，（ST 9（RIRDC）、ST 13（RIRDC）、ST 27（RIRDC）、ST 50（RIRDC）和ST 741（RIRDC）以及新的序列类型ST 322（RIRDC））。在绵羊分离株中鉴定的ST仅在小型反刍动物中检测到，表明对这些宿主的适应，而在猪分离株中鉴定的基因型先前已在多个宿主中鉴定，因此它们不限于猪。绵羊和猪分离株之间的基因型和VAG谱的差异表明它们可能代表不同的多杀性巴氏杆菌亚群。
Pasteurella multocida is a pathogen causing disease in a wide range of hosts including sheep and pigs. Isolates from ovine pneumonia were characterized by MLST (Multi-host and RIRDC databases) and virulence-associated gene WAG) typing and compared with porcine isolates. Ovine and porcine isolates did not share any STs as determined by both schemes and exhibited different VAG profiles. With the Multi-host database, sixteen STs were identified among 43 sheep isolates with two STs (ST50 and ST19) comprising 53.5% of the isolates, and seven MLST genotypes (ST3, ST11 and ST62 included 75% of the isolates) among the 48 pig isolates. The most frequent VAG profile among sheep isolates was tbpA +/toxA + (69.8% of isolates) and pfhA + (62.5%) and hgbB + (33.3%) among pig isolates. Representative ovine and porcine isolates of those STs identified by the Multi host scheme were further typed using the RIRDC scheme. Seven STs were identified among the ovine isolates (ST95(RIRDC), ST131(RIRDC), ST203(RIRDC), ST320(RIRDC), ST324(RIRDC), ST321(RIRDC), and STRIRDC), with the latter four sequence types being new STs identified in this study, and six STs (ST9(RIRDC), ST13(RIRDC), ST27(RIRDC), ST50(RIRDC), and ST741(RIRDC) and a new sequence type ST322(RIRDC)) among the porcine isolates. STs identified among ovine isolates have been detected exclusively in small ruminants, suggesting an adaptation to these hosts, while the genotypes identified among pig isolates have been previously identified in multiple hosts and therefore they are not restricted to pigs. The differences in genotypes and VAG profiles between ovine and pig isolates suggest they could represent different subpopulations of P. multocida.