Involvement of opioid receptors in the CGRP-induced antinociception in the nucleus accumbens of rats
Involvement of opioid receptors in the CGRP-induced antinociception in the nucleus accumbens of rats
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阿片受体参与 CGRP 诱导的大鼠伏核镇痛作用
DOI：
10.1016/j.brainres.2010.07.042
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发表时间：
2010-09-24
期刊：
影响因子：
2.9
通讯作者：
Yu, Long-Chuan
中科院分区：
文献类型：
--
作者：
Li, Lixin;Wang, Xuebing;Yu, Long-Chuan
研究表明，降钙素基因相关肽(CGRP)在大鼠伏隔核(NAC)的伤害性感受调制中起重要作用。本研究旨在探讨阿片受体在降钙素基因相关肽(CGRP)诱导的大鼠NAc抗伤害性感受中的作用。NAC内注射CGRP可显著延长大鼠对伤害性热刺激和机械刺激的后爪撤退潜伏期(HWL)。有趣的是，在NAc内注射阿片受体拮抗剂纳洛酮后，CGRP的抗伤害效应被抑制，提示阿片受体参与了CGRP在NAc的抗伤害效应。NAc内注射多阿片受体(MOR)拮抗剂β-FNA和kappa-阿片受体(KOR)拮抗剂Nor-binaltorphimine(Nor-BNI)可减弱CGRP的抗伤害性作用，但不能被DOR拮抗剂那曲吲哚所减弱。在本研究中，我们还证明了在大鼠的NAc中，CGRP诱导的抗伤害感受与吗啡诱导的抗伤害感受没有显著差异。本研究结果表明，Mu阿片受体和kappa阿片受体均参与CGRP诱导的大鼠NAc的抗伤害性感受。(C)2010爱思唯尔B.V.保留所有权利。
It has been demonstrated that calcitonin gene-related peptide (CGRP) plays important roles in the modulation of nociception in the nucleus accumbens (NAc) of rats. The present study is performed to explore the possible involvement of opioid receptors in the CGRP-induced antinociception in the NAc of rats. Intra-NAc administration of CGRP induces significant increases in the hindpaw withdrawal latency (HWL) to noxious thermal and mechanical stimulation in rats. Interestingly, the CGRP-induced antinociceptive effects are inhibited by following intra-NAc injection of the opioid receptor antagonist naloxone, suggesting that the opioid receptors are involved in the CGRP-induced antinociception in the NAc of rats. Furthermore, the CGRP-induced antinociception is attenuated by intra-NAc injection of muopioid receptor (MOR) antagonist beta-funaltrexamine (beta-FNA) and kappa-opioid receptor (KOR) antagonist nor-binaltorphimine (nor-BNI), but not by delta-receptor (DOR) antagonist naltrindole. In the present study, we also demonstrated that there was no significant difference between the CGRP-induced antinociception and the morphine-induced antinociception in the NAc in rats. The results of the present study demonstrate that both mu- and kappa-opioid receptors are involved in the CGRP-induced antinociception in the NAc of rats. (C) 2010 Elsevier B.V. All rights reserved.