STORAGE AND SECRETION PROCESSES IN THE SPORE OF GIGASPORA-MARGARITA BECKER AND HALL AS REVEALED BY HIGH-PRESSURE FREEZING AND FREEZE-SUBSTITUTION
STORAGE AND SECRETION PROCESSES IN THE SPORE OF GIGASPORA-MARGARITA BECKER AND HALL AS REVEALED BY HIGH-PRESSURE FREEZING AND FREEZE-SUBSTITUTION
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DOI：
10.1111/j.1469-8137.1994.tb03991.x
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发表时间：
1994-09-01
期刊：
影响因子：
9.4
通讯作者：
MENDGEN, K
中科院分区：
文献类型：
--
作者：
BONFANTE, P;BALESTRINI, R;MENDGEN, K
采用高压冷冻法对丛枝菌根真菌Gigaspora margarita Becker and Hall的孢子进行冷冻，并用冷冻取代法制备用于透射电镜观察。低温技术改善了孢子细胞质的超微结构保存，揭示了玛格丽塔芽孢萌发过程中的储存和分泌过程。在水合和/或发芽孢子中的储存结构被确定为属于三种不同的类别：(i)被膜限制的脂滴，（ii）特殊液泡内的蛋白质样体，以及（ii）糖原颗粒。在胚管分化过程中，观察到丰富的膜质系统。它由粗内质网、小管和气球状高尔基等价物组成，它们产生与电子致密颗粒相关的囊泡。颗粒似乎与壁结合在一起了。结果表明，真菌孢子中的分泌和储存过程与酵母或植物细胞中的相似。
Spores of Gigaspora margarita Becker and Hall, an arbuscular mycorrhizal fungus, were cryofixed using high-pressure freezing and prepared for transmission electron microscopy by freeze substitution. The cryotechniques improved the ultrastructural preservation of the spore cytoplasm and revealed storage and secretion processes in the germinating spores of G. margarita. The storage structures in the hydrated and/or germinating spores have been identified as belonging to three different classes: (i) lipid droplets limited by a membrane, (ii) protein-like bodies inside specialized vacuoles, and (ii) glycogen particles. During germ-tube differentiation, an abundant membranous system was observed. It consists of rough ER, tubules and balloon-like Golgi equivalents, which produce vesicles associated with electron-dense granules. The granules seem to be incorporated into the wall. The results suggest that secretion and storage occur in the fungal spore during events comparable to those in yeasts or plant cells.