Infection and pathogenesis of Huaiyangshan virus (a novel tick-borne bunyavirus) in laboratory rodents
Infection and pathogenesis of Huaiyangshan virus (a novel tick-borne bunyavirus) in laboratory rodents
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DOI：
10.1099/vir.0.041053-0
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发表时间：
2012-06-01
影响因子：
3.8
通讯作者：
Zhang, Yong-Zhen
中科院分区：
文献类型：
--
作者：
Chen, Xiao-Ping;Cong, Mei-Li;Zhang, Yong-Zhen
一种新的蜱传布尼亚病毒（淮扬山病毒，HYSV），引起出血热样疾病，最近在中国被报道。迄今为止，还没有动物实验来研究其发病机制。为了建立HYSV热的动物模型，将新生和成年小鼠、大鼠和金黄地鼠脑内或腹腔内接种HYSV。新生大鼠和新生小鼠，尤其是昆明种小鼠，表现出高度易感性。值得注意的是，死于HYSV感染的KM小鼠在肝脏中出现大面积坏死，而在其他器官中未观察到明显的病理变化。PCR和免疫组织化学分析的尸检材料检测到HYSV抗原和RNA在几乎所有的器官，表明系统性感染。我们的数据表明，HYSV可引起新生小鼠和新生大鼠的致死性感染，并伴有明显的肝脏病理损伤。这种动物模型可能有助于了解人类HYSV感染的发病机制。
A novel tick-borne bunyavirus (Huaiyangshan virus, HYSV), which causes haemorrhagic fever-like disease, has recently been reported in China. So far no animal experiments have been performed to study its pathogenesis. Towards developing an animal model for HYSV fever, newborn and adult mice and rats and golden hamsters were inoculated intracerebrally or intraperitoneally with HYSV. Newborn rats and newborn mice, especially Kunming (KM) mice, appeared highly susceptible. Remarkably, the KM mice that died of the HYSV infection developed large necrotic areas in the liver, while no obvious pathological changes were observed within the other organs. PCR and immunohistochemical analyses of the post-mortem material detected both HYSV antigen and RNA in almost all organs, indicating a systemic infection. Our data demonstrate that HYSV can cause a lethal infection of both newborn mice and newborn rats with apparent pathological damage of the liver. This animal model may help to understand the pathogenesis of the HYSV infection in humans.