Isoproterenol decreases protein permeability in edematous isolated rabbit lungs: estimation of PS and sigma.
Isoproterenol decreases protein permeability in edematous isolated rabbit lungs: estimation of PS and sigma.
复制标题
异丙肾上腺素降低水肿离体兔肺中的蛋白质通透性：PS 和 sigma 的估计。
DOI：
10.1152/jappl.1994.77.1.325
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Minnear,FL
中科院分区：
文献类型：
--
作者：
Kreienberg,PB;Vincent,PA;Bell,DR;Saba,TM;Minnear,FL
本研究的目的是确定-肾上腺素能激动剂异丙肾上腺素减轻肺水肿的能力是否通过渗透性和/或血流动力学机制发生。在离体灌注兔肺中，分别通过测量毛细管过滤系数（Kf）和125i标记白蛋白的经血管清除率来评估血管屏障对液体和蛋白质运动的限制特性。利用线性或非线性通量方程对白蛋白清除率与经血管流体通量进行回归分析，以估计透性-表面积积（PS）和反射系数（sigma）。还评估了动脉、毛细血管和静脉压力和阻力、体重增加和干湿体重比。异丙肾上腺素（8 ng.ml-1）min-1)减弱花生四烯酸（4mg）引起的流体通量、干湿比、白蛋白清除率和PS的增加以及sigma的降低。异丙肾上腺素对Kf的增加没有影响，在四组中毛细血管压力和流体通量之间没有相关性。回归分析显示，与线性通量方程相比，非线性通量方程提供的PS和sigma估价值更准确地描述了研究组之间白蛋白清除率的统计差异。这些发现表明，异丙肾上腺素通过改变血管屏障的蛋白质通透性，减弱了水肿肺中增加的白蛋白经血管通量。
The objective of the present study was to determine whether the ability of the beta-adrenergic agonist isoproterenol to attenuate pulmonary edema occurs via a permeability and/or hemodynamic mechanism. In isolated perfused rabbit lungs, the restrictive property of the vascular barrier to the movement of fluid and protein was assessed by measurements of the capillary filtration coefficient (Kf) and the transvascular clearance of 125I-labeled albumin, respectively. Regression analysis of albumin clearance vs. transvascular fluid flux was performed to estimate the permeability-surface area product (PS) and the reflection coefficient (sigma) by use of the linear or nonlinear flux equation. Arterial, capillary, and venous pressures and resistances, weight gain, and the wet-to-dry weight ratio were also assessed. Isoproterenol (8 ng.ml-1.min-1) attenuated the arachidonic acid (4 mg)-induced increases in fluid flux, wet-to-dry weight ratio, albumin clearance, and PS and the decrease in sigma. Isoproterenol had no effect on the increase in Kf, and there was no correlation between capillary pressure and fluid flux in any of the four groups. Regression analysis revealed that the non-linear flux equation provided estimates of PS and sigma that more accurately described the statistical differences in albumin clearance among the groups studied than the linear flux equation. These findings demonstrate that isoproterenol attenuated the increased transvascular flux of albumin in edematous lungs by modifying the protein permeability of the vascular barrier.