Plasmid donor affects host range of promiscuous IncP-1β plasmid pB10 in an activated-sludge microbial community
Plasmid donor affects host range of promiscuous IncP-1β plasmid pB10 in an activated-sludge microbial community
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DOI：
10.1128/aem.71.9.5309-5317.2005
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发表时间：
2005-09-01
影响因子：
4.4
通讯作者：
Top, EM
中科院分区：
文献类型：
--
作者：
De Gelder, L;Vandecasteele, FPJ;Top, EM
环境中多重耐药质粒的水平转移导致耐药病原体问题日益严重。尽管质粒宿主细胞是质粒发挥功能的主要环境，但尚未研究质粒供体对质粒在微生物群落中传播的细菌范围的可能影响。在这项研究中，我们表明，广泛的主机范围内的一个活性污泥微生物群落的质粒的宿主范围的供体菌株的影响，各种交配条件和隔离策略增加了检测到的transconjugants的多样性。为了检测接合转移体，质粒pB 10用lacp-rfp标记，而rfp表达在供体中被染色体lacI（q）抑制。通过16 S rRNA基因部分序列分析，确定了306个接合子的遗传性。transconjugants属于15个属的α-和γ-变形菌。在与供试菌Pseudomonasputida SM 1443、Ralstonia eutropha JMP 228和Sinorhizobium meliloti RM 1021的不同交配中获得的接合子的系统发育多样性存在显著差异。例如，在污泥中与S.苜蓿RM 1021包括8个属，这些属在用另外两个供体获得的接合子中不存在。我们的研究结果表明，在微生物群落中，混杂质粒转移到的宿主的谱可以受到它转移的供体的强烈影响。
Horizontal transfer of multiresistance plasmids in the environment contributes to the growing problem of drug-resistant pathogens. Even though the plasmid host cell is the primary environment in which the plasmid functions, possible effects of the plasmid donor on the range of bacteria to which plasmids spread in microbial communities have not been investigated. In this study we show that the host range of a broad-host-range plasmid within an activated-sludge microbial community was influenced by the donor strain and that various mating conditions and isolation strategies increased the diversity of transconjugants detected. To detect transconjugants, the plasmid pB10 was marked with lacp-rfp, while rfp expression was repressed in the donors by chromosomal lacI(q). The phylogeny of 306 transconjugants obtained was determined by analysis of partial 16S rRNA gene sequences. The transconjugants belonged to 15 genera of the alpha- and gamma-Proteobacteria. The phylogenetic diversity of transconjugants obtained in separate matings with donors Pseudomonas putida SM1443, Ralstonia eutropha JMP228, and Sinorhizobium meliloti RM1021 was significantly different. For example, the transconjugants obtained after matings in sludge with S. meliloti RM1021 included eight genera that were not represented among the transconjugants obtained with the other two donors. Our results indicate that the spectrum of hosts to which a promiscuous plasmid transfers in a microbial community can be strongly influenced by the donor from which it transfers.