Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit
Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit
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DOI：
10.1016/j.bbamem.2017.01.027
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发表时间：
2017-05-01
影响因子：
3.4
通讯作者：
Mingarro, Ismael
中科院分区：
文献类型：
--
作者：
Bano-Polo, Manuel;Martinez-Garay, Carlos A.;Mingarro, Ismael
易位子相关蛋白 (TRAP) 复合物与内质网易位子密切相关，用于在真核细胞中插入或易位新合成的蛋白质。 TRAP 复合体由三个单跨亚基和一个多跨亚基组成。我们通过体外和细胞培养中的糖基化作图和绿色荧光蛋白融合研究了多跨 TRAP-gamma 亚基的膜插入和拓扑结构。结果表明，TRAP-gamma 具有四个跨膜 (TM) 片段、Nt/Ct 胞质取向，并且疏水性较低的 TM 片段仅在全长蛋白质的细胞环境中才能有效插入膜中。 (C) 2017 Elsevier B.V. 保留所有权利。
Translocon-associated protein (TRAP) complex is intimately associated with the ER translocon for the insertion or translocation of newly synthesised proteins in eukaryotic cells. The TRAP complex is comprised of three single-spanning and one multiple-spanning subunits. We have investigated the membrane insertion and topology of the multiple-spanning TRAP-gamma subunit by glycosylation mapping and green fluorescent protein fusions both in vitro and in cell cultures. Results demonstrate that TRAP-gamma has four transmembrane (TM) segments, an Nt/Ct cytosolic orientation and that the less hydrophobic TM segment inserts efficiently into the membrane only in the cellular context of full-length protein. (C) 2017 Elsevier B.V. All rights reserved.