Ultrasonic-assisted extraction of sinomenine from Sinomenium acutum using magnetic ionic liquids coupled with further purification by reversed micellar extraction
Ultrasonic-assisted extraction of sinomenine from Sinomenium acutum using magnetic ionic liquids coupled with further purification by reversed micellar extraction
复制标题
使用磁性离子液体超声辅助提取青藤碱并通过反胶束萃取进一步纯化
DOI：
10.1016/j.procbio.2017.04.030
复制
发表时间：
2017-07-01
影响因子：
4.4
通讯作者：
Tan, Zhijian
中科院分区：
文献类型：
--
作者：
Li, Qiao;Wu, Shuanggen;Tan, Zhijian
磁性离子液体具有与常规离子液体相同的性质，而且在永磁磁场作用下具有可回收性。因此，基于咪唑阳离子和铁（III）阴离子的MIL用于超声辅助提取（UAE）青风藤中的青藤碱（SIN），在本研究中的第一次。在单因素试验中考察了提取条件，并采用响应面法对影响提取效果的主要因素MIL浓度、液料比和超声时间进行优化。在最佳工艺条件下，提取率可达10.57mg/g。采用AOT/异辛烷体系对SIN的MIL粗提物进行反胶束萃取（RME）。经RME纯化后，SIN的回收率和纯度分别达到81.3%和82.6%。这种MIL-UAE和RME的混合方法用于SIN的提取和纯化是有效的，快速的，并且具有放大潜力。
Magnetic ionic liquids (MILs) have the same features as conventional ionic liquids (ILs), moreover they possess the potentiality of being recovered under the action of permanent magnetic field. Thus, MILs based on imidazolium cations and iron(III) anions were used for the ultrasonic-assisted extraction (UAE) of sinomenine (SIN) from Sinomenium acutum for the first time in this study. The extraction conditions were investigated in the single factor experiments, then the major factors of MIL concentration, liquid/solid ratio, and ultrasonic irradiation time were optimized by response surface methodology (RSM). The extraction yield reached 10.57 mg/g under the optimized conditions. The crude MIL extract of SIN was further purified by reversed micellar extraction (RME) using AOT/isooctane system. The recovery and the purity of SIN reached 81.3% and 82.6%, respectively after purification by RME. This hybrid method of MIL-UAE and RME used for the extraction and purification of SIN was effective, rapid, and potential for scale-up.