A preparation of bacterial outer membrane with osmium tetroxide and uranyl acetate co-stain enables improved structural determination by transmission electron microscopy.
A preparation of bacterial outer membrane with osmium tetroxide and uranyl acetate co-stain enables improved structural determination by transmission electron microscopy.
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用四氧化锇和乙酸双氧铀共染色制备细菌外膜，可以改进透射电子显微镜的结构测定。
DOI：
10.1093/jmicro/dfad027
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Greathouse,KLeigh
中科院分区：
文献类型：
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作者：
Sheikh,Aadil;Zechmann,Bernd;Sayes,ChristieM;Taube,JosephH;Greathouse,KLeigh
生物纳米颗粒，如细菌外膜囊泡（OMV），通常通过透射电子显微镜（TEM）进行表征。在这项研究中，我们报告了一种新的方法来制备OMV的TEM成像。为了保持囊泡的形状和结构，我们开发了一种双重固定方案，包括四氧化锇孵育，然后用醋酸双氧铀进行负染色。将四氧化锇与乙酸双氧铀组合导致亚50 nm囊泡的保存和改善的形态稳定性，通过TEM增强了基于脂质的纳米颗粒的表征。
Biological nanoparticles, such as bacterial outer membrane vesicles (OMVs), are routinely characterized through transmission electron microscopy (TEM). In this study, we report a novel method to prepare OMVs for TEM imaging. To preserve vesicular shape and structure, we developed a dual fixation protocol involving osmium tetroxide incubation prior to negative staining with uranyl acetate. Combining osmium tetroxide with uranyl acetate resulted in preservation of sub-50 nm vesicles and improved morphological stability, enhancing characterization of lipid-based nanoparticles by TEM.