Smooth muscle cell functionality on collagen immobilized polycaprolactone nanowire surfaces.
Smooth muscle cell functionality on collagen immobilized polycaprolactone nanowire surfaces.
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DOI：
10.3390/jfb5020058
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发表时间：
2014-05-08
影响因子：
4.8
通讯作者：
Popat KC
中科院分区：
文献类型：
--
作者：
Leszczak V;Baskett DA;Popat KC
抑制平滑肌细胞（SMC）增殖和保持分化状态是血管疾病管理、避免和进展的重要方面。了解SMCs和所涉及的生物材料之间的相互作用对于成功的植入是至关重要的。在这项研究中，我们开发了胶原蛋白固定的纳米结构表面与控制阵列的高纵横比纳米线的生长和维护的人主动脉平滑肌细胞。纳米线表面由聚己内酯制成，并用胶原蛋白固定。本研究的目的是揭示SMCs如何与胶原固定的纳米结构相互作用。结果表明，纳米结构和胶原蛋白固定表面上的细胞粘附显著更高;然而，纳米结构表面上的SMC表现出更细长的表型。MTT的减少在纳米线（NW）和胶原固定的NW（colNW）表面上显著较低，表明纳米结构表面上的SMC可以分化并缓慢分裂。扫描电子显微镜结果显示，纳米结构表面上的SMC更细长，并且细胞与表面上的纳米特征相互作用。在提供分化线索后，特异于收缩性SMC表型的重链肌球蛋白和钙调蛋白在胶原固定表面上上调。这些结果表明，纳米形貌影响细胞粘附，增殖，以及细胞伸长，而胶原蛋白固定化表面极大地影响细胞分化。
Inhibition of smooth muscle cell (SMC) proliferation and preservation of a differentiated state are important aspects in the management, avoidance and progression of vascular diseases. An understanding of the interaction between SMCs and the biomaterial involved is essential for a successful implant. In this study, we have developed collagen immobilized nanostructured surfaces with controlled arrays of high aspect ratio nanowires for the growth and maintenance of human aortic SMCs. The nanowire surfaces were fabricated from polycaprolactone and were immobilized with collagen. The objective of this study is to reveal how SMCs interact with collagen immobilized nanostructures. The results indicate significantly higher cellular adhesion on nanostructured and collagen immobilized surfaces; however, SMCs on nanostructured surfaces exhibit a more elongated phenotype. The reduction of MTT was significantly lower on nanowire (NW) and collagen immobilized NW (colNW) surfaces, suggesting that SMCs on nanostructured surfaces may be differentiated and slowly dividing. Scanning electron microscopy results reveal that SMCs on nanostructured surfaces are more elongated and that cells are interacting with the nano-features on the surface. After providing differentiation cues, heavy chain myosin and calponin, specific to a contractile SMC phenotype, are upregulated on collagen immobilized surfaces. These results suggest that nanotopography affects cell adhesion, proliferation, as well as cell elongation, while collagen immobilized surfaces greatly affect cell differentiation.