The role of transforming growth factor-α on mucin overproduction in eosinophilic chronic rhinosinusitis
The role of transforming growth factor-α on mucin overproduction in eosinophilic chronic rhinosinusitis
复制标题
转化生长因子-α 对嗜酸性慢性鼻-鼻窦炎粘蛋白过量产生的作用
DOI：
10.1159/000333794
复制
发表时间：
2011
期刊：
影响因子：
3.1
通讯作者：
Sakaida H,Takeuchi K
中科院分区：
文献类型：
--
作者：
Ishinaga H;Shah SA;Sakaida H,Takeuchi K
目的：嗜酸粒细胞性慢性鼻窦炎（ECRS）被认为是一种难治性、难治性疾病，具有粘液粘稠、长期鼻充血、嗅觉丧失以及继发于细菌感染的间歇性急性加重的症状。在本研究中，我们调查了哪种生长因子与ECRS粘蛋白过量产生密切相关。方法：我们采用荧光免疫组织化学分析来评估ECRS患者鼻组织中TGF-α表达是否上调。我们还使用荧光素酶报告质粒在 HM3-MUC5AC 细胞和 A549 细胞中检查了 MUC5AC 转录，以评估 TGF-α 在人上皮细胞中的作用。 结果：发现与正常患者和非嗜酸性 CRS 患者相比，ECRS 患者粘膜下组织中的 TGF-α 免疫反应性显着增加。 TGF-α与TNF-α协同作用，通过ERK信号通路上调人上皮细胞中MUC5AC的表达。结论：我们的研究结果表明，TGF-α在ECRS患者的上气道中高表达，并深度参与粘液分泌过多。
Objectives:Eosinophilic chronic rhinosinusitis (ECRS) is considered a refractory and intractable disease with thick mucus production, long-term nasal congestion, loss of sense of smell and intermittent acute exacerbations secondary to bacterial infections. In this study, we investigated which growth factor is deeply involved in the mucin overproduction in ECRS.Method:We employed fluorescence immunohistochemical analysis to evaluate whether or not TGF-α expression was upregulated in the nasal tissue of ECRS patients. We also examined MUC5AC transcription using a luciferase reporter plasmid in HM3-MUC5AC cells and A549 cells in order to assess the role of TGF-α in human epithelial cells.Results:TGF-α immunoreactivity was found markedly increased in the submucosal tissue in the ECRS patient compared with that of a normal patient and with noneosinophilic CRS. TGF-α synergized with TNF-α to upregulate MUC5AC expression in human epithelial cells through the ERK signaling pathway.Conclusion:Our results demonstrated that TGF-α was highly expressed in the upper airway tract in ECRS patients and is deeply involved in mucus hypersecretion.