The Characterization of Fish (Tilapia) Collagen Sponge as a Biomaterial
The Characterization of Fish (Tilapia) Collagen Sponge as a Biomaterial
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DOI：
10.1155/2015/957385
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发表时间：
2015-06
影响因子：
3.3
通讯作者：
Kohei Yamamoto;Y. Yoshizawa;K. Yanagiguchi;T. Ikeda;Shizuka Yamada;Y. Hayashi
中科院分区：
文献类型：
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作者：
Kohei Yamamoto;Y. Yoshizawa;K. Yanagiguchi;T. Ikeda;Shizuka Yamada;Y. Hayashi
对于支架制造，来自海洋产品的生物活性天然有机材料的效用是有用的和不可或缺的，作为牛胶原蛋白的替代品。用于支架应用的鱼胶原蛋白的最弱特征是其低变性温度（），表明鱼胶原蛋白在哺乳动物体内的稳定性差。我们专注于热带鱼罗非鱼作为产生临床支架的候选者。本研究的目的是确定罗非鱼I型端肽胶原（TAC）的生物医学应用。此外，物理和结构特性进行了研究和评估作为海绵形式上的支架。分析不同浓度{0.5%、1.0%和2.0%（v/v）}的TAC溶液。差示扫描量热法测得TAC的热稳定性为35-36 ° C。扫描电子显微镜结果表明，TAC海绵的孔径（90 - 160 μ m）对于细胞增殖是可接受的。多孔海绵的拉伸强度在0.01 - 0.07MPa的范围内。这些发现表明，从罗非鱼制备的TAC海绵是作为支架的候选者之一。特别推荐1.0%（v/v）浓度的TAC溶液，以有利于制备和处理溶液以及海绵形成。
For scaffold manufacturing, the utility of bioactive natural organic materials derived from marine products is useful and indispensable as an alternative to bovine collagen. The weakest feature of fish collagen for scaffold application is its low degeneration temperature (), indicating poor stability of fish collagen in mammals in vivo. We have focused on the tropical fish tilapia as a candidate for generating a clinical scaffold. The aim of this study was to confirm the of tilapia type I atelocollagen (TAC) for biomedical application. Furthermore, the physical and structural properties were investigated and evaluated as a scaffold on a sponge form. Different concentrations {0.5%, 1.0%, and 2.0% (v/v)} of TAC solution were analyzed. Differential scanning calorimetry showed that the of TAC was 35-36°C. The scanning electron microscopy results indicated that the pore size (90–160 μm) of TAC sponges is acceptable for cell proliferation. The tensile strength of porous sponges was in the range of 0.01–0.07 MPa. These findings indicate that the TAC sponge prepared from tilapia is one of candidates as a scaffold. The 1.0% (v/v) concentration of TAC solution is especially recommended to be advantageous for preparing and handling the solution and for sponge formation.