Molecular heterogeneity of gamma delta T-cell antigen receptors expressed by CD4- CD8- T-cell clones from normal donors: both disulfide- and non-disulfide-linked receptors are delta TCS1+.
Molecular heterogeneity of gamma delta T-cell antigen receptors expressed by CD4- CD8- T-cell clones from normal donors: both disulfide- and non-disulfide-linked receptors are delta TCS1+.
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来自正常供体的 CD4-CD8-T 细胞克隆表达的 γ δ T 细胞抗原受体的分子异质性：二硫键连接和非二硫键连接的受体都是 δ TCS1。
DOI：
10.1073/pnas.86.7.2326
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发表时间：
1989
影响因子：
11.1
通讯作者：
Platsoucas,CD
中科院分区：
文献类型：
--
作者：
Seki,H;Nanno,M;Chen,PF;Itoh,K;Ioannides,C;Good,RA;Platsoucas,CD
我们研究了正常供体外周血淋巴细胞产生的 T 细胞克隆上表达的 γ δ T 细胞抗原受体 (TCR) 的分子异质性。在 γ 链水平上观察到广泛的分子异质性，在 δ 链水平上也观察到了较小程度的分子异质性。二硫化物和非二硫化物 gamma delta TCR 均已被发现，并使用具有相似分子量（范围：41-43 kDa）的不同 γ 链。相比之下，在源自免疫缺陷疾病患者外周血的 T 细胞系上表达的 55-60 kDa 的 γ 链，在源自正常供体外周血的 T 细胞克隆上未发现。这些 T 细胞克隆上表达的 δ 链的分子量为 37 kDa，并且是二硫键或非二硫键连接的。使用抗 delta TCS1 和抗 TCR delta 1 单克隆抗体在这些克隆中鉴定出显着的 delta 链异质性。所有测试的克隆都是 TCR delta 1+，而只有 25% 的克隆是 delta TCS1+。抗 δ TCS1 单克隆抗体对来自正常供体的不同 T 细胞克隆的二硫键和非二硫键连接的 γ δ TCR 进行染色和免疫沉淀。
We investigated the molecular heterogeneity of gamma delta T-cell antigen receptors (TCR) expressed on T-cell clones generated from peripheral blood lymphocytes of normal donors. Extensive molecular heterogeneity was seen at the gamma-chain level and, to a lesser extent, at the delta-chain level. Both disulfide and non-disulfide gamma delta TCR were found and use different gamma chains with similar molecular masses (range, 41-43 kDa). In contrast, gamma chains of 55-60 kDa, which are expressed on T-cell lines derived from the peripheral blood of patients with immunodeficiency disorders, were not found on T-cell clones derived from the peripheral blood of normal donors. delta chains expressed on these T-cell clones had a molecular mass of 37 kDa and were either disulfide or nondisulfide linked. Significant delta-chain heterogeneity was identified in these clones using the anti-delta TCS1 and the anti-TCR delta 1 monoclonal antibodies. All clones tested were TCR delta 1+, whereas only 25% of the clones were delta TCS1+. The anti-delta TCS1 monoclonal antibody stained and immunoprecipitated both disulfide- and non-disulfide-linked gamma delta TCRs from different T-cell clones from normal donors.